intracellularly
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But the interest protein was still expressed intracellularly. In this study, we have constructed expression vectors. Bovine IL2 has been expressed in Pichia pastoris, but in a fused protein form existing intracellularly. The effection of signal peptide and its procession on foreign protein secretion have been studied.
总之,本研究通过不同构建策略的表达载体的构建,实现了目的蛋白在真核体系中的表达,但以融合蛋白的形式存在于胞内,通过不同信号肽和信号肽加工位点的构建,探讨了影响目的蛋白有效分泌的原因。
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There was partial secretion of PC F139V, most of the protein molecule was not secreted and degraded intracellularly.
结论分泌障碍和细胞内降解是PC C64W和PC F139V导致PC缺陷症的原因,PC K150d对突变体蛋白的分泌无明显影响。
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Clark electrode method and reverse phase HPLC were used to detect oxygen consumption and ATP content intracellularly, respectively.
分别用Clark电极法和反相高压液相法测定细胞内氧耗量和ATP含量。