hexamer

hexamer的基本解释

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六聚物

与hexamer相关的网络例句 [注：此内容来源于网络，仅供参考]

Reerse transcription was performed with 3 g of total RNA, random hexamer (Amersham Bioscience, Buckinghamshire, United Kingdom), and Superscript II reerse transcriptase at conditions recommended by the manufacture, and then incubated with 1l of RNase at 37oC for 20 minutes.

再根据生产说明介绍的条件，从中选取3克RNA，随机六聚物（英国白金汉郡Amersham生物科学公司生产的）以及逆转录标记II进行逆转录，用1LRNA酶(Initrogen公司生产)在37度的环境中孵化20分钟。

The binding enthalpy change, the binding molar ratio and the binding equilibrium constant of harmine with hexamer are obtained.

而对d〓与潜在的抗肿瘤药物骆驼蓬碱的相互作用研究，已得到harmine与d〓结合的摩尔结合焓变，结合常数与结合摩尔比。

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

It is possible that the linker proteins located in the center of APC discs may play a important role in stabilizing this loose APC hexamer.

有可能位于APC盘中心的连接蛋白对稳定这个APC疏松的六聚体起重要作用。

Through the comparison analysis for two MD simulation data, it was found that for the system with Zn2+ ions and phenol, the conformational flexibility of insulin hexamer was decreased.

用分子动力学模拟方法设计了两个模拟时间为600 ps的对比计算机模拟实验，研究了R6态的胰岛素六聚体在水溶液中的构象柔性。

与hexamer相关的网络解释 [注：此内容来源于网络，仅供参考]

hexamer：六聚体

该分子能将胆色素原合酶(porphobilinogen synthase PBGS)限制在不活跃状态,PBGS是一种所有细胞都要用到的酶,其功能形态是由8个完全相同组分构成的"八聚体"(octamer),PBGS对所有生命形式都很重要,它使细胞能利用能量. 而PBGS另一种形态则是"六聚体"(hexamer).

hexamer：六聚物

Hexalithus 六骸面 | hexamer 六聚物 | hexamethyldisilazane 六甲基二硅氮烷