glycerin trilaurate
- glycerin trilaurate的基本解释
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[化] 甘油三酯, 甘油三十二酸酯, 三月桂精
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Methods:(1) Silica column chromatography and LH-20 were used in phytochemistry study.(2) Based on the extraction ratio of arctiin, the best extraction procedure was acheived under the direction of L9(34) orthogonal experimental design. From 10 types of resins, the one with the maximal binding ability for Arctii was selected and silica-column choramatograpy was used in its seperation and purification procedure.(3) The animal diabetic experimental model in Wistar rats was established with streptozotocin and many biochemical factors were used to indicate the pharmacological effects of arctiin, including glycosylated hemoglobin, serum creatinine, seralbumin, monoxide nitrogen, glycerin trilaurate, cholesterol, HDL-ch, LDL-ch, Blood urea nitrogen, creatinine, SOD, ET, MDA, total Urine protein and albuminuria.
(1)采用硅胶柱层析、聚酰胺层析、LH-20等方法对牛蒡子的化学成分进行研究;(2)采用正交实验设计方法,以牛蒡子苷为指标,确定了牛蒡子乙醇回流提取的最佳工艺;对10种类型大孔吸附树脂进行筛选,筛选出对牛蒡子苷吸附效果最佳的树脂;采用硅胶柱层析法,对牛蒡子苷进一步分离纯化;(3)建立实验性糖尿病大鼠模型,以GLU、TC、TG、HDL-ch、LDL-ch、TB、ALB、BUN、CREA、GHbAlc、NO、SOD、ET、MDA和尿液中TB、ALB为指标,研究牛蒡子苷药理活性;(4)以差速消化法纯化Wistar大鼠的主动脉内皮细胞并进行传代培养,测定牛蒡子苷对高糖条件下RAECs活性、RAECs释放LDH、MDA和NO的变化以及RAECs表达内皮型一氧化氮合酶表达的影响。