get used to...

与get used to...相关的网络例句 [注：此内容来源于网络，仅供参考]

When the mind apprehends the essence of a thing (quod quid est; tò tí en enai), the external object is perceived without the particular notes which attach to it in nature, and it is not yet marked with the attribute of generality which reflection will bestow on it.

当铭记apprehends的本质的东西（狱吏嚼1-5 ；德州仪器恩enai ），外部对象被认为没有特别注意到它重视它的性质（中存在singularibus ），这是没有标明属性一般性的反映将赋予它

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

The rain is knockin at my heart and I can't see the sun no more I can't live without you my sun if I'm ever gonna get back picture this You got a kid in a room his hands sweatin and shakin while he's lacin his shoes the excitement's buildin up for what's comin up soon To get there and do what he was born to do He's listenin to the beats He's trying to get in the zone He's gotta take control and make every moment his own just a little bit tired he is a little bit scared but he's so focused he won't show it when he's finally there Cuz now he's out on the floor And he's catchin the pass slashin to the glass, movin so fast, right to the hole, they don't know how to stop him and he keeps on droppin 2's and 3's by the second and the crowd is rockin so they knock him knock him down to the ground before he's crowned as the best He takes a deep breath before he gets up and looks around Everything's in slow motion The crowd is an ocean Only one minute left The rain is knockin at my heart and I can't see the sun no more I can't live without you me sun if I'm ever gonna get back, get back, get back The rain is knockin at my heart and I can't see the sun no more Ican't lice without you my sunif I'm ever gonna get back Imagine A little girl in the stands the kid's her older brother and she's his number one fan He's always lookin out for her and she looks up to him and she wants nothin more than for her brother to win But the truth is that there's someone on the other team Someone who stole her heart Someone who holds her dreams They kiss for the 1st time just the other evening Her brother's 23 but her lover's number 16 The drama is set the whole scene is intense she doesn't know which one to cheer for and she's gettin upset 23 on the chase cuz they're at each other's necks and what happens next Her boyfriend steals the ball and checks the kid in the chest 16 on the break Brother goes in the air with brother up in his face They come crashing to the dirt Tears about to burst When the game is over who she gonna run to first, yo The rain is knockin at my door and I can't see the sun no more I'ma hafta fight my way if I'm ever gonna get back, get back, get back The rain is knockin at my door and I can't see the sun no more I'ma hafta fight my way if I'm ever gonna get back 有时感觉阻碍太多但我还是为了我的梦想而奋斗 Nothin's gonna stop me Ey yo this game of life is one big struggle We gotta hustle just to make it everyday to fight the fears we facin and grab those dreams we chasin cuz there ain't no competition when we rise to the occasion c'mon The rain is knockin at my door and I can't see the sun no more I'ma hafta fight my way if I'm ever gonna get back, get back, get back The rain is knockin at my door and I can't see the sun no more I'ma hafta fight my way if I'm ever gonna get back 注:IN可以看作是ING如FACIN可以看作facing

雨是敲入我的心我看不到太阳不再我不能没有你我的太阳如果我曾经去买回来此图片你有一个孩子在一个房间里他的手sweatin和沙金，而他的拉欣他的鞋兴奋的buildin了什么的，很快就要来要到那里做什么，他出生做他listenin的节拍他试图在该地区获得他得采取控制和利用自己的每一刻只是有点累了他有点害怕但他是如此专注，他将不显示它的时候，他终于有现在的Cuz他在地上他的捉通行证 slashin的玻璃，居无定得那么快，有权向孔，他们不知道如何阻止他他不断droppin 2的和3的由第二摇滚和人群所以他们会把他会把他撞倒在地之前，他的加冕为最佳他深吸了一口气，才起身环顾四周一切都在慢动作人群是一个海洋仅1分钟雨是敲入我的心我看不到太阳不再我不能没有你我的太阳如果我曾经去买回来，回来，回来雨是敲入我的心我看不到太阳不再没有你我的sunif Ican't虱子我曾经去买回来想像看台上的一个小女孩这个孩子是她的哥哥，她是他的头号球迷他总是在找谁了她和她抬起头来他和她想没什么比她的哥哥更赢得但是，事实是，对其他团队的人有人谁偷了她的心有人谁拥有她的梦想他们只是亲吻对方的第一时间为晚上她的弟弟，但她的爱人23的16号该剧设置整个场面激烈她不知道哪一个加油，她的gettin不高兴 23大通他们的Cuz在对方的脖子和接下来会发生什么她的男朋友抢断球，并检查孩子的胸部 16个突破哥哥和弟弟去他的脸在空中他们来到崩溃的污垢即将爆裂泪当比赛结束谁，她要去跑第一，溜溜雨是敲着我的门我看不到太阳不再我是打我的方式hafta 如果我曾经去买回来，回来，回来雨是敲着我的门我看不到太阳不再我是打我的方式hafta 如果我曾经去买回来有时感觉阻碍太多但我还是为了我的梦想而奋斗没办法，拢不惊安永哟这样的生活游戏是一个大斗争我们得喧嚣这只是为了日常战斗的恐惧，我们都会面临的两个并抓住那些梦想，我们查辛没有的Cuz没有竞争当我们挺身而出来吧雨是敲着我的门我看不到太阳不再我是打我的方式hafta 如果我曾经去买回来，回来，回来雨是敲着我的门我看不到太阳不再我是打我的方式hafta 如果我曾经去买回来

与get used to...相关的网络解释 [注：此内容来源于网络，仅供参考]

get used to...=become accustomed to：习惯于

in general:一般地、大体上 | get used to...=become accustomed to...:习惯于... | complain about:抱怨

get used to sth. / doing sth：变得习惯于

21、 浓烈的滋味:strong flavours | 22、 变得习惯于:get used to sth. / doing sth. | 23、 开阔你的视野:expand your horizon

get used to sth. / doing sth：习惯于

4. But great changes have already taken place in China recently.但是近(dan shi jin)来中国已发生了巨大的变化. | 1. get used to sth./ doing sth. 习惯于...... | 2. as a matter of fact 事实上

get used to sth. / doing sth：适应

3. adjust to sth. 调节 | 4. get used to sth./doing sth. 适应... | 5. take part in / participate in 参加

to get used to sb./sth：习惯某人/某事

to have a chance to do sth. 有机会做某事 | to get used to sb./sth. 习惯某人/某事 | to meet one's need 满足某人需求