fragment ['frægmənt]

fragment的基本解释

n.

碎片, 破片, 断片, 片段

• The glass fell to the floor and broke into fragments.
• 玻璃杯落在地上碎成破片。

• She dropped the bowl on the floor and it broke into fragments.
• 她把碗掉在地上，碗破成碎片。

fragment的同义词

n. division · part · section · segment · fraction · portion

与fragment相关的网络例句 [注：此内容来源于网络，仅供参考]

The results showed that the PCR fragment length of root-knot samples was 768bp in cowpea(RKN-1), tomato(RKN-2), balsam pear(RKN-3), Astragalus adsurgens (RKN-19)and rose(RKN-4) from Beijing, peanut fromWeifang (RKN-7) Yantai(RKN-6) and Jiaozhou(RKN-5) in Shandong, tomato from Hexian(RKN-9), cucumber from Suzhou (RKN-8) and Platycodon grandiflorum from Taihe(RKN-11) in Anhui and two kinds of tomato species(RKN-22,23) from China Central Agricultural University; the PCR fragment length of root-knot samples was 769bp in balsam pear from Ganyu(RKN-10) in Jiangsu, tomato from Kunming(RKN-12) in Yunnan, Langfang (RKN-17)in Hebei, Chengdu(RKN-18) in Sichuan and Suzhou(RKN-21) in Anhui and cucumber from Sixian(RKN-20) in Anhui; the PCR fragment length of root-knot samples was 772bp in guava(RKN-13) and pawpaw (RKN-15) from Hainan; the PCR fragment length of the other two samples was both 766bp in tomato(RKN-24,25) from China Central Agricultural University, and the PCR fragment length of root-knot nematodes in cucumber from Hangzhou in Zhejiang(RKN-16) and pepper from Anding (RKN-14)in Hainan was 767bp and 869bp, respectively.

结果表明，北京密云的豇豆、番茄、苦瓜，北京植保站月季，山东胶州，烟台和潍坊的花生，安徽和县的番茄，宿州的黄瓜和太和的桔梗，北京畜牧所沙打旺以及中国农业大学两个番茄品种(RKN-23和RKN-25)上的根结线虫种群的PCR扩增片段长度为768bp；江苏赣榆苦瓜，云南昆明，河北廊坊，安徽宿州和四川成都番茄以及安徽泅县黄瓜上根结线虫种群的PCR扩增片段长度为769bp；海南安定木瓜和石榴上根结线虫种群的PCR扩增长片段度为772bp；中国农业大学另外两个番茄(RKN22和RKN-24)品种上的根结线虫种群的PCR扩增片段长度为766bp；而浙江杭州黄瓜和海南安定胡椒上的根结线虫种群的PCR扩增长度分别为767bp和869bp。

Total RNAs from KAx-3 cells and AK127 cells(developed for 14h) were isolated. After the reverse transcription and PCR reaction, two distinct differential fragments were acquired., fragment A was from KAx-3 cells and fragment C was from AK127 cells. After retriving and reamplifying the differentially expressed fragments, white-blue plaqueselection, the fragments were purified. Northern blot proved that fragment A was from KAx-3 cells and fragment C was from AK127 cells. The results of sequencing and researching for NCBI database have been showed: part sequence of fragment A shows 91% similarity to the gene encoding DhkA, 92% similarity to the gene encoding DhkF, 91% similarity to the gene encoding STATc, 97% similarity to the homoeobox gene encoding protein. These genes play important part in controlling cell differentiation and cell proportion in Dictyostelium discoideum.

本研究通过提取盘基网柄菌发育14小时的野生型KAx-3细胞和突变型AK127细胞的总RNA，运用mRNA差异显示法分离出了两条明显的差异表达片段，其中片段A来自野生型KAX-3细胞，片段C来自突变型Ak127细胞；并通过凝胶回收差异片段、对差异片段进行再次PCR、蓝白斑筛选克隆、提取质粒、酶切电泳纯化差异片段；接着进行Northern杂交的结果表明，片段A只与野生型KAx-3细胞的总RNA有杂交信号，片段C只与突变型AK127细胞的总RNA有杂交信号，这就排除了差异片段假阳性的可能；最后通过测序，搜索NCBI BLAST数据库发现：片段A的小部分序列与编码组氨酸激酶DhkA基因中一段序列的相似性高达91%，与编码组氨酸激酶DhkF基因中的一段序列相似性高达92%，与编码STATc蛋白基因的一段序列相似性达91%，以及与编码同源框蛋白的基因中的一段序列相似性达97%，这些基因在盘基网柄菌细胞分化和细胞比例调控过程中起着相当重要的作用，这些数据进一步说明了突变细胞不能完成发育的原因。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

与fragment相关的网络解释 [注：此内容来源于网络，仅供参考]

fragment：碎片

AIX4以上的日志型文件结构中引入"碎片"(Fragment)概念. 目的:减少磁盘空间的浪费.

fragment：片段

对爱情的追寻与对暴力的恐惧,正是皮娜鲍什创作一贯的主题. 她很快在编舞领域崭露头角. 1968年她的处女作>(Fragment)未引起轰动,但次年便凭>(ImWindderZeit)在当年欧洲新生代编舞家大赛中夺魁,一举成名.

fragment：片断

例如,片断(fragment)的采用可以作为一种变形手段,因为"片断可以带来它自身之外的丰富性和含义"[19],而且还暗示了一种潜在的连续性. 在文丘里理论的影响下,建筑表皮的变形手法在风格上的后现代主义建筑,尤其是美国后现代建筑中得到了突出表现,

fragment：破片

克瑞沙克(Kresak)认为Phaethon是彗星破片(fragment)的残留物(remnant),短周期彗星是一原本很大的天体之残留物,此天体在不断地失去质量后仍保有足够的大小;彗核的破裂物或许能解释在轨道上四散的流星体.

fragment：abbr. frag; 碎片叫(如