fig banana
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Fig.1 SHEE cultured on coverslide, the living cells were growing in single layer with rich cytoplasm, the nuclei were uniform in size with a nucleolus ph ×400 Fig.2 SHEE had a nucleus with ellipse shape, large nucleolus and the cytoplasm contained mitochondria and tonofibrilEM ×10 000 Fig.3 SHEE was spherical in shape, with pseudopods attached on petri dish and abundant villi on cell surface SEM ×5 000 Fig.4 Same as in Fig.3, cell attached on petri dish, appeared stellate or polygonal in shape, with abundant pseudopods and cytoplasmic processes. Protrusive nuclear region in central part of the cell had more micro-villi SEM ×5 000 Fig.5 Chromosomes of SHEE cells belonged to diploidy type Giemsa ×1 000 Fig.6 The SHEE cells of stained in dark brown by Ki67 immunohistochemistry were the proliferative cells Immunohistochemistry ×400 Fig.7 In SHEE cell culture, the nucleus stained red or pink by PI was dead cell, the green nucleus was living cell Fluorescent ×400 Fig.8 The cell labeled by TdT was apoptotic cell in which the chromatin of nucleus condensed in block, a pyknotic nucleus in the upper right conner was seen TdT labeled ×400
图1 SHEE培养在盖坡片上,活细胞单层生长,胞浆较丰富,细胞核大小一致,有核仁×400 图2 SHEE培养细胞细胞核椭圆形,核仁较大,胞浆有较丰富的线粒体和张力原纤维EM ×10 000 图3 SHEE细胞呈球状,有伪足贴壁,表面有密集微绒毛SEM ×5 000 图4 同上细胞贴壁,呈星状或多角形,有丰富伪足和胞浆突,核区隆起有较多微绒毛SEM ×5 000 图5 SHEE细胞染色体仍属二倍体Giemsa染色×1 000 图6 SHEE细胞Ki67免疫组织化学染棕黄色为增殖细胞×400 图7 SHEE培养细胞出现死细胞,胞核和胞浆PI染色呈红色或淡红色,蓝色细胞核为活细胞荧光显微镜×400 图8 细胞TdT标记阳性为凋亡细胞,染色质凝集呈块状,右上角有一固缩细胞核TdT标记×400
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Appressoria were latent in intercellular cleft and were latent until banana fruit were harvested.The development process of conidia of Colletotrichum musae on fruit was not distinct from foliage and stalk.Two pairs of PCR primers were designed according to the two especial fragment (357bp and 206bp) of Colletotrichum musae. Banana tissue culture seedling genomic DNA, banana anthracnose pathogen genomic DNA, mango anthracnose pathogen genomic DNA, rubber anthracnose pathogen genomic DNA,watermelon anthracnose pathogen genomic DNA, banana crown rot pathogen genomic DNA, stylo anthracnose pathogen genomic DNA, watermelon Fusarium wilt pathogen genomic DNA were extracted using SDS method.
根据香蕉炭疽菌的两个特异片段(分别为357bp和206bp),设计两对引物:采用SDS法分别提取了香蕉组培苗基因组DNA、香蕉果实炭疽菌弱致病株Z_1基因组DNA、香蕉果实炭疽菌强致病株Z_4基因组DNA、芒果炭疽菌基因组DNA、橡胶炭疽菌基因组DNA、柱花草炭疽菌基因组DNA、西瓜炭疽菌基因组DNA、香蕉冠腐病菌基因组DNA、西瓜枯萎病菌基因组DNA;以上述基因组DNA为模板对特异片段进行PCR验证,证明357bp的片段为Colletotrichum musaes所特有,可以用此片段进行香蕉果实炭疽病的分子检测试验,。
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For instance, the "banana" sign is caused by downward displacement of the cerebellum, with a resultant banana shape, and it also leads to effacement of the cisterna magna Fig. 10-19A-B and Fig.
例如,"香蕉"征是由小脑向下移位,造成了香蕉样的外形所引起,它还导致小脑延髓池的消失(图10-19A-B和图10-19-C)。
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banana fig:香蕉干
"香蕉","banana" | "香蕉乾","banana fig" | "金带梅鲷","banana fish"