expressing
- expressing的基本解释
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[变形] express的现在分词
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vt.
表达, 表示, 表露
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adj.
明确的, 急速的, 特殊的
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adv.
乘快车, 以快递方式
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n.
快车, 快递, 专使
- 相似词
- 更多 网络例句 与expressing相关的网络例句 [注:此内容来源于网络,仅供参考]
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RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .
结果 成功构建突变人CD59的真核细胞表达系统:运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞:用含有400ug/mlG418的F12培养基培养14天,筛选出稳定阳性表达克隆,RPMI1640培养基扩增获得稳定表达细胞株,并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02,表达率分别为53.7%、54.5%;应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株;RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株,裂解细胞得到CD59蛋白质;通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达;50mM核糖培养72小时,获得突变人CD59糖化细胞株,BCECF染料释放试验结果显示,PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低,未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。
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The second chapter is on the category of pragmatic quantity which are expressed by modality, Three "shi" structures expressing pragmatic quantity are discussed: the adverbs of degree "henshi\',"zuishi" structures expressing subjectivity; the rhetoric question structure of "bushi" expressing different kinds and scales of pragmatic quantity; and the compound sentence structure of "bushi不是 A, jiushi B expressing emphasizing.
着重考察了三种表达语用量的&是&字结构:能进行主观表达的程度副词&很是、最是&、能表达语用量的不同类型和等次的特定句型&不是&型反问句、能表达强调功能的&不是A,就是B&复句格式。
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The strategies used for indirectness recognition are generalized in terms of false expressing, uninformative expressing, expressing in unusual manner, and setting language activity.
为了使听话人识别间接言语,说话人可以采取提供虚假信息、零信息、不寻常表达方式和设定言语活动类型的策略。
- 更多网络解释 与expressing相关的网络解释 [注:此内容来源于网络,仅供参考]
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Expressing anxiety:表示焦虑
教师指导学生归纳本课中有关表示焦虑(Expressing anxiety)的常用语句(见日常交际用语部分). We found him to be dishonest.我们发现他不诚实. (to be)
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Expressing anxiety:二十八、表示焦虑
二十七、提醒Reminding | 二十八、表示焦虑Expressing anxiety | 二十九、表示惊奇Expressing surprise
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Expressing anxiety:表示担心
3 Making introductions做介绍 | 4 Expressing anxiety表示担心 | 5 Apologizing道歉
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Expressing anxiety:表现焦虑
Make sure that everything is OK now.看一看是不是一切都好了 | 28.表现焦虑 Expressing anxiety | (1)What's wrong?怎么啦?出什么弊病啦?
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Expressing Sympathy:表示同情
17.Expressing Anger表示愤怒 | 19.Expressing Sympathy表示同情 | 20.Making Complaints抱怨
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