distinguish between
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The result would be used in tobacco breeding.4 SRAP technology is used to study the genetic differences among different types of tobacco, including flue-cured tobacco, oriental tobacco, maryland tobacco, burley tobacco, and air/sun cured tobacco, five pairs of primer combinations which can amplify characteristic bands has been screened. Among the five combinations, two of them (SF25*SR2 and SF3*SR26) can distinguish air-cured tobacco including maryland tobacco and Burley tobacco from the other cultivated forms; SF20*SR14 can distinguish sun cured tobacco including oriental tobacco and sun-cured tobacco; SF16*SR14 can distinguish flue-cured tobacco and air-cured tobacco, and SF22*SR14 can distinguish DBJ599 with specisl aroma and the other flue-cured tobacco.5 8 varieties , which represent the different types of tobacco, are divided into 3 groups. I: G-28, II:DBJ599、MD40、By21, III: Basma、Sumsun、XNS、QCT. The cluster analysis result es consistant with experience evatuation.6 Anorthogonal design was used to optimize a ILP-PCR system with 5 factors (DNA, Mg2+, dNTP, primer and Taq polymerase) at 4 levels.The result showed the optimized ILP-PCR system for Nicotiana tabacum L. was: 2uL10×PCRbuffer, 20ng template DNA, Mg2+ 2.0mmol/L, dNTP 150umol/L, primer 0.3umol/L, Taq DNA polymerase 1 U in a total of 20uL reactionsolution.
研究结果可作为烤烟与香料烟类型间的杂种鉴定利用的实验与理论依据。4、利用SRAP标记技术,研究和分析了烤烟、晒烟、晾烟、白肋烟、香料烟等不同栽培类型间的基因型差异,初步筛选出了能够在分子水平上反映不同烟草类型差异的特征性标记带:晾烟MD40、Ky17、Burley21、TN90基因型共有的特征带有两对引物组合,分别为SF25*SR2和SF3*SR26;晒烟小牛舌、青梗、小花青和香料烟Sumsun、komotini Basma、Xanthi Basma共有的特征带引物组合为SF20*SR14;NC89、K326、G-28、大白筋和MD40、Ky17、Burley21、TN90共有的特征带引物组合为SF16*SR14,烤烟中大白筋599的特征带引物组合SF22*SR14.5、利用61对SRAP引物组合,研究了普通烟草不同类型间的亲缘关系,将普通烟草类型间8个不同供试材料划分为3类别。I:烤烟类型G-28II:白肋烟类型(MD40、By21、大白筋599III:香料烟类型(Basma、Sumsun、小牛舌、千层塔)。
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Furthermore, out of 497 fAFLP markers, 80 special bands were found to be able to distinguish the four groups from each other and may be applied for germplasm characterization and molecular assistant classification of Meretrix clam.4 Molecular classification of two species of Meretrix clam based on fAFLP and ITS sequences4.1 The results of fAFLP maker analysis of S, G and W showed that each group had their own specific loci among which there were 53 special loci in W group, much more than those of S group (14) and G group (21). Among the 53 loci, nine were all dominant loci. These unique loci could be taken as molecular markers to distinguish W from other groups. The genetic similarity indexes and distance matrix between S and G groups were 0.9585 and 0.0424 respectively, but the genetic similarity indexes and distance matrix between W group and S or G group was 0.7939 or 0.7941, and 0.2308 or 0.2305 respectively. The results revealed that significant difference existed between W and S or G groups in molecular genetic structure. The phylogenetic trees by the methods of UPGMA and NJ also indicated that S and G populations were very closely related, while W population was a relatively independent cluster, lying beyond the species which S and G belong to.4.2 The internal transcribed spacer region of the rDNA from S group, G group and W group were PCR amplified and sequenced. The results showed that the size of ITS ranged between 1266-1269bp in W group, while those in G and S groups were 1614bp and 1520bp respectively. The GC content ranged 62.32-62.62% in W group while it was 61.77% in G group. The genetic distances between three populations of W group were 0.001~0.003, but it was 0.110 or 0.147 respectively between W group and G group or S group. Phylogenetic trees by NJ method also showed that G group was very closely related to S group, while W group was a relatively independent cluster.
在457个总扩增位点中找出了53个W的特有位点,远多于S群体(14)和G(21)群体,而且在53个特有位点中有9个出现频率为100%的位点,这些位点可以作为区分其它2个群体的特征性标记;S– G群体特有的位点有112个,其中有4个位点出现频率为100%,可作为S– G群体区别于W群体的特征性标记。S群体和G群体间的遗传相似性系数为0.9585,遗传距离只有0.0424,在NJ和UPGMA法构建的亲缘关系的树状图上均首先聚在一起,说明二者的亲缘关系很近,应属于种内群体间的关系;而W与S和G的遗传相似性系数均较小(0.7939和0.7941),相对遗传距离很大而且十分相近(0.2308和0.2305),在亲缘关系树状图上单独分出一支,也表明W与S和G群体间的亲缘关系较远。4.2 ITS序列比较分析通过对白壳文蛤、山东文蛤和广西文蛤的ITS序列扩增电泳、PCR-RFLP分析和ITS序列分析发现,W的ITS序列长度在1266-1269 bp,而S和与G的ITS序列总长度分别为1520 bp和1614 bp;从ITS1和ITS2长度来看,W分别为739-741 bp和316-317 bp,S为895 bp和414 bp,G为987 bp和416 bp;而从ITS碱基组成来看,W的GC含量在62.32-62.62%之间,而G群体为61.77%。W的3个壳色不同群体间的遗传距离仅0.001、0.002和0.003,S与G群体间的遗传距离是0.010,说明W群体内变异很小,而S与G群体间已出现明显的遗传分化,但还均属于种内群体间的遗传变异;而W与G和S的遗传距离分别达到0.110、0.147,两个类群差异显著,已远超出种内群体间的遗传变异。
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The fiber length has only little influence on the basic density within the growth rings, and significant correlation at 0.01 levels was found between the basic density and the fiber length among the different rings. Only slight negative correlation was found between the basic density and the fiber width within the growth rings, but significant positive correlation at 0.01 levels was indicated between the basic density and the fiber width among the growth rings, contrary to that of fiber length. It was demonstrated that significant positive correlations at 0.01 levels between the basic density and fiber double wall thickness, fiber length to width ratio and double wall thickness to diameter ratio, significant negative correlations at 0.01 levels between the basic density and fiber diameter and diameter to width ratio, only slight negative correlation between the basic density and fiber width both in the same growth rings and among the different growth rings. No significant correlation was found between the basic density and the vessel morphological features, nor was the tissue proportion in the same growth rings. But among the different rings, it was found there was significant positive correlation at 0.01 levels between the basic density and the fiber proportion among the different rings, and significant negative correlation at 0.01 levels between the basic density and vessel-elements proportion and ray proportion, only slight negative correlation between the basic density and the parenchym proportion. Significant or no significant negative correlation was found between the basic density and the microfibril angle in the same growth rings, but significant negative correlation was found between the basic density and the microfibril angle among the different growth rings.
生长轮内纤维长度对基本密度的影响不大,而在不同生长轮间纤维长度与基本密度达极显著正相关,纤维宽度与此相反,同一生长轮内纤维宽度与基本密度极显著负相关,不同生长轮间只有微弱负相关;基本密度与纤维双壁厚、长宽比、壁腔比在生长轮内和生长轮间均呈极显著正相关,而与胞腔直径、腔径比均呈极显著负相关,仅与纤维宽度呈微弱的负相关;导管形态对基本密度的影响不显著;同一生长轮内组织比量对基本密度的影响也不显著,但不同生长轮间基本密度与纤维比量呈极显著正相关,与导管比量和木射线比量呈极显著负相关,与轴向薄壁细胞比量仅呈不显著负相关;生长轮内基本密度与微纤丝角呈显著或不显著负相关,但在生长轮间这种负相关达到极显著水平。
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distinguish between:把...和...区别,弄清
die out消失,灭绝 | distinguish between把...和...区别,弄清 | do away with废除,去掉
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distinguish between:分辨,区分
pull down 拆除;推翻 | distinguish between 分辨,区分 | come to terms with 达成协议;妥协,习惯于
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distinguish between:区别
distingue 高尚的 | distinguish between 区别 | distinguish from 区别
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distinguish between:区分
die out 灭绝 | distinguish between 区分 | do away with 废除
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to distinguish between:分辨,区分
to come to terms [with] 达成协议;妥协,习惯于 | to distinguish between 分辨,区分 | to pull down 拆除;推翻
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