differentially
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Methods The differentially expressed genes between liver tumor and non-tumor tissue were cloned by using suppression subtractive hybridization. Differentially expressed genes were sequenced and analized for homology in Gene Bank. In situ hybridization was performed to identify special expression of differentially expressed genes in livre tumor and non-tumor tissues.
方法通过抑制消减杂交法克隆土拨鼠HBV引发的肝癌组织和非癌组织中差异表达的基因;对差异表达基因进行DNA序列测定后于Gene Bank内分析其同源性;应用原位杂交确定差异基因在肝癌组织和非癌组织中的特异性表达。
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The number of differentially expressed genes was 71 at 24h, and 62 at 72h. The metabolisic gene class was the majority among all the differentially expressed genes, and the next was transportational、differentiation and adhesional gene class by turns.1.2. RT-PCR verification of the known HIF-1 targeted genes and proliferation related genesIn the 131 differentially expressed genes, we found 7 known HIF-1 targed genes (ldha、aldoa、eno1、gapdh、tpi1、pgk1、bnip3) and 5 genes (dnaja2、ccnb1、tgfβ2、ccng1、rgs7) which were related to proliferation and cell cycle.
对差异表达的基因进行基因功能分类发现:代谢类基因最多,其次是运输类基因、细胞分化及黏附类基因。1.2 RT-PCR验证HIF-1靶基因和增殖相关的基因由于我们的前期实验证明了低氧促NSC增殖是有HIF-1参与的,我们对131个差异表达基因中,7个已知的HIF-1靶基因(ldha、aldoa、eno1、gapdh、tpi1、pgk1、bnip3)和5个与细胞周期和增殖相关的基因(dnaja2、ccnb1、tgfβ2、ccng1、rgs7)做RT-PCR验证。
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Expression patterns of genes differentially expressed in starch metabolism and cell cycle during endosperm development7 DAP kernals, 15 DAP endosperms and 25 DAP endosperms of B73, ae/wx and sh1 were used to study the expression patterns of 18 differentially expressed genes in sugar metabolism and 9 differentially expressed genes in cell cycle. We found that the expression patterns of Pul, SBEⅡa, UGPase, SUS3, SSⅡa in sh1 and Sh2-1, SUS3, SSⅡa in ae/wx were altered, revealing that these genes may interact with each other.
碳水化合物代谢基因和细胞周期基因随胚乳发育的变化以B73、ae/wx、sh1授粉后7天种子、15天和25天胚乳为材料,分析18个糖代谢差异基因和9个细胞周期调节相关的差异基因的表达模式,研究发现sh1突变体中Pul、SBEⅡa、UGPase、SUS3和SSⅡa基因表达模式发生改变,ae/wx突变体中Sh2-1、SUS3和SSⅡa基因表达模式发生改变,揭示了突变体对这些基因的巨大影响,反映了它们可能存在相互作用。
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differentially:区别地
differentialfluxionalfluxionary 微分的 | differentially 区别地 | differentiateandanalysediscriminate 辨析
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differentially:差别/区别地
differential /差别的/特定的/微分的/两路线运费差额/工资差额/ | differentially /差别/区别地/ | differentiate /辨别/区别/区分/
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joining-up differentially:差接
joining by rabbets 槽舌接合 | joining-up differentially 差接 | joining-up in parallel 并接
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differentially expression:差异表达
差异表达基因:specific expressed gene | 裂殖酵母:gene that is preferentially expressed in cotton fiber | 差异表达:differentially expression
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differentially value-equivalent:可微分值等价
differentially compound-wound machine 差复励电机 | differentially value-equivalent 可微分值等价 | differentially-compound wound motor 差复激电动机
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