date fig
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Fig.1 SHEE cultured on coverslide, the living cells were growing in single layer with rich cytoplasm, the nuclei were uniform in size with a nucleolus ph ×400 Fig.2 SHEE had a nucleus with ellipse shape, large nucleolus and the cytoplasm contained mitochondria and tonofibrilEM ×10 000 Fig.3 SHEE was spherical in shape, with pseudopods attached on petri dish and abundant villi on cell surface SEM ×5 000 Fig.4 Same as in Fig.3, cell attached on petri dish, appeared stellate or polygonal in shape, with abundant pseudopods and cytoplasmic processes. Protrusive nuclear region in central part of the cell had more micro-villi SEM ×5 000 Fig.5 Chromosomes of SHEE cells belonged to diploidy type Giemsa ×1 000 Fig.6 The SHEE cells of stained in dark brown by Ki67 immunohistochemistry were the proliferative cells Immunohistochemistry ×400 Fig.7 In SHEE cell culture, the nucleus stained red or pink by PI was dead cell, the green nucleus was living cell Fluorescent ×400 Fig.8 The cell labeled by TdT was apoptotic cell in which the chromatin of nucleus condensed in block, a pyknotic nucleus in the upper right conner was seen TdT labeled ×400
图1 SHEE培养在盖坡片上,活细胞单层生长,胞浆较丰富,细胞核大小一致,有核仁×400 图2 SHEE培养细胞细胞核椭圆形,核仁较大,胞浆有较丰富的线粒体和张力原纤维EM ×10 000 图3 SHEE细胞呈球状,有伪足贴壁,表面有密集微绒毛SEM ×5 000 图4 同上细胞贴壁,呈星状或多角形,有丰富伪足和胞浆突,核区隆起有较多微绒毛SEM ×5 000 图5 SHEE细胞染色体仍属二倍体Giemsa染色×1 000 图6 SHEE细胞Ki67免疫组织化学染棕黄色为增殖细胞×400 图7 SHEE培养细胞出现死细胞,胞核和胞浆PI染色呈红色或淡红色,蓝色细胞核为活细胞荧光显微镜×400 图8 细胞TdT标记阳性为凋亡细胞,染色质凝集呈块状,右上角有一固缩细胞核TdT标记×400
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Offer for the grant of option The board is pleased to advise you that pursuant to a resolution passed at a meeting of the board held on 8th March 2007,the board has resolved to grant you the "Offer"(which is still subject to listing of committee of the stock exchange approving the granting of the listing of and permission to deal in the shares which may fall to be issued pursuant to the exercise of the options under the Pre-IPO Share Option Scheme) at a consideration of HK$1.00 an option pursuant to the Pre-IPO Share Option Scheme to subscribe for 40000 shares at an exercise price of 85% of final HK dollar price per share at which the shares are to be subscribed for /purchased and issued/ sold pursuant to the global offering of the shares in the initial public offering in or around March 2007 at any time during the six-year period commence from the expiry of twelve months from the listing dateprovided that the maximum number of shares in which you are entitled to subscribe for by exercising the option shall not exceed 25%of the total number of option shares during the period from expiry of the first anniversary of the listing date to the date immediately before the second anniversary of the listing date :and 25%of the total number of option shares during the period from expiry of the second anniversary of the listing date to the date immediately before the third anniversary of the listing date ;and 25%of the total number of option shares during the period from expiry of the third anniversary of the listing date to the date immediately before the fourth anniversary of the listing date ;and 25%of the total number of option shares during the period from expiry of the fourth anniversary of the listing date to the date immediately before the fifth anniversary of the listing date save that any of the outstanding and unexercised option at the end of each of the aforesaid vesting periods may be rolled over to the next vesting period and exercisable during the option period ,and the option shall,in any case ,lapse where you cease to be employed by the company or its subsidiaries for whatere reason or otherwise pursuant to the terms of the Pre-IPO Share Option Scheme.
登录后回答可以获得经验值奖励,并可以查看和管理所有的回答。登录|1.Offer的期权该委员会很高兴地通知您,根据是在对2007年3月8日举行的董事会会议上通过一项决议,该委员会决定授予您"要约"(依然受在联交所上市委员会批准给予上市,并准许其在交易的股票可能下跌发出依据根据首次公开招股前购股权计划之购股权获行使)在审议为港币1.00元的选择根据首次公开招股前购股权计划认购40000股为85港元,最终每股%美元的价格行使价格,股票要认购/购买并发行/出售依照全球发售的股份在最初在2007年3月左右上市期间的任何六年期开始从十二个月上市日期提供的届满时间,在你有权认购股份的最大数量行使在紧接上市日期2周年选项不得超过(在从上市日期一周年期间届满的日期一)25的选择权股份总数%:及在从该上市日期2周年期间届满之日起的25选择股份总数%,紧接上市三周年之日;25的选择权股份总数%,与从上市日期四周年期间届满的日期紧接上市日期五周年,并保存该悬而未决的和未执行的选择任何在最后上述的归属期间每年可结转到下一个归属期期间内行使的期权,而选择应在任何情况下,失误,你不再是由公司或whatere或其他原因及其附属公司雇用根据该条款前首次公开售股购股权Scheme。
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Fig 1 Negative control Female umbilical cord sample has no Y-specific signal after in situ hybridization with the biotinylated Y-repeated sequence DNA probe PY3.4 Fig 2 Positive control Flow-sorted male umbilical cord cells hybridized to Y-specific DNA probe PY3.4,Every cell contains a Y-specific signal Fig 3 Fetal cells sorted from maternal blood Flow-sorted cells from a pregnant woman at 20 weeks of gestation hybridized to Y-specific DNA probe PY3.4,containing a Y-specific signal Fig 4 The result of polymerase chain reaction Lane 1:male umbilical cord NRBCs sorted by FITC-conjugated anti-monoclonal glycophorin A;Lane 2:sample of 2;Lane 3:male umbilical cord NRBCs sorted by FITC-conjugated anti-CD36;Lanes 4-5:samples of 1 and 3;Lane 6:50 cells of male;Lane 7:5 cells of male;Lane 8:200ng male DNA(positive+control);Lane 9:nonpregnant female DNA(negative+control);Lane 10:ΦX174 HaeⅢ Maker,271bp:amplified band of Y-specific gene SRY;383bp:amplified band of human β-globin gene
图1 阴性对照女性脐带血标本,经与生物素标记的Y-染色体重复序列DNA探针原位杂交后未见Y-染色体特异信号图2 阳性对照分选的男性脐血细胞经与Y-特异DNA探针杂交后,每一细胞都含有Y-染色体特异信号图3 母体外周血中分选的胎儿细胞从一位妊娠20周的孕妇外周血中分选出的细胞经与Y-特异DNA探针杂交细胞中含有Y-染色体特异信号图4 聚合酶链反应结果 1:GPA-FITC单抗分选男胎脐血NRBCs;2:2号标本;3:CD36-FITC单抗分选男胎脐血NRBCs;4、5:1、3号标本;6:50个男性细胞标本;7:5个男性细胞标本;8:200ng男性DNA;9:未孕女性DNA;10:ΦX174 HaeⅢ标准,271bp:为SRY基因扩增带;383bp:为人β-珠蛋白基因扩增带内参照
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botree fig;peepul tree:菩提木
(树皮)布榕木 bark cloth fig;Natal fig | 菩提木 botree fig;peepul tree | 野生无花果 wild fig tree;sycamore
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bark cloth fig;Natal fig:(树皮)布榕木
马(达加斯加)榕木 manoko | (树皮)布榕木 bark cloth fig;Natal fig | 菩提木 botree fig;peepul tree
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F.elastica Roxb Indiarubber fig,Caoutchouc,Indiarubber Tree:印度橡皮树
无花果 F.carica L Fig | 印度橡皮树 F.elastica Roxb Indiarubber fig,Caoutchouc,Indiarubber Tree | 黄葛树 F.lacor Buch.-Ham Bigleaf Fig