construct form
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Transcriptional activity of ACL recombinants normalized by Renilla was significant difference with pGL3-Basic expect for construct -27 and -15 (P<0.01). Construct -919 contains highest activity. 3 times reduction of transcriptional activity from-919 to -679bp indicated that negative regulation factors located probably in this region. The activity started on construct -73 suggested the basal promoter activity was located within the -73 to +77bp region; Transcriptional activity of IDHβrecombinants was not significantly different between the recombinant -58 and pGL3-Basic. The activity started on construct -82, decreasing with the length of the fragment up to -164 in despite of a bit of fluctuation, and kept increasing from construct -164 up to -279. Thus, the basal promoter activity was located within the -82 to +16bp region, whereas the upstream 197bp conferred maximal transcriptional activity.
采用5'端缺失策略,结合启动子预测结果,分别构建了8个ACL基因和19个IDH3β基因启动子区重组子,将其转染猪PK15细胞系,并利用荧光素酶双报告基因系统检测了它们的活性,结果表明:在所构建的猪ACL基因5侧翼序列的8个重组子中,除pGL-ACL27和pGL-ACL15外,其它重组子的荧光素酶活性与阴性对照均达到极显著水平(P<0.01),表明它们均具有启动子活性,pGL-ACL919活性最强,到pGL-ACL679活性下降了将近3倍,说明从-919bp到-679bp区域可能存在负的调控位点,从pGL-ACL621到pGL-ACL73活性有小幅度下降,到pGL-ACL27活性降到与阴性对照水平无显著差异,表明维持该启动子的基本活性区域位于-73bp到+77bp之间;IDH3β基因启动子活性开始于pGL-IB82,然后虽然有些波动,但是活性一直下降直到-164bp处,之后活性又开始升高,直到-279bp处活性达到最高,这些表明维持该启动子的基本活性区域位于-82bp到+16bp之间,从-82到-279之间的179bp区域具有最高的启动子活性。
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Adopt ASP. NET 2003 is regarded as the developing instrument, the design of storehouse of the data follows 3 normal form :, have designed the coding schedule of goods and materials mainly, tombarthite product structure catalogue, basic information slip of the job centre, route form of the craft , project code of the expenses, journal ledger of the cost of goods, subsidiary ledger of the cost of goods, department's form, measurement unit form, the supplies inventory safeguards the counting type structure form, real expenses form, share the proportion form in power of kinetic energy, salary expenses share proportion form, amortization charge share proportion , maintain expenses share proportion , test expenses share proportion , main frame left menu tree form , material price list ,etc. data list
采用ASP 。NET 2003作为开发工具,数据库设计遵循第三范式,符合第二范式的条件,每个非关键字属性都仅由关键字决定,而且一个非关键字属性不能仅仅是对另一个非关键字属性的进一步描述(即一个非关键字属性值不依赖于另一个非关键字属性值)。,主要设计了物资编码表、稀土产品结构目录表、工作中心的基本情况表、工艺路线表、费用项目编码、产品成本总帐、产品成本明细帐、部门表、计量单位表、物料清单维护数型结构表、实际费用表、动能功率分摊比例表、工资费用分摊比例表、折旧费用分摊比例、维护费用分摊比例、化验费用分摊比例、主框架左菜单树表、材料价格表等数据表。
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The paper analyses material transportation problem and give the solvable methods; analyses delivery vehicle route problem, and then construct its corresponding mathematical model and present a heuristic algorithm based on C—W algorithm; analyses the optimum scheduling problem of trucks, and then construct its corresponding mathematical model and put forward a new algorithm; analyses the Postal Transportation Problem which will perhaps be used in supply chain of manufacturing system, and then construct its corresponding mathematical model and present an approximation algorithm.
分析了制造系统供应链中供需平衡、供需不平衡、有转运等各种情况下的物资调运问题,给出了求解方法;分析了制造系统供应链配送作业中的配送车辆路线问题,建立了相应的数学模型,提出了一种基于C-W算法的启发式算法,该算法较好地解决了有时间约束的配送问题;分析了制造系统供应链中的货运卡车优化调度问题,建立了相应的数学模型,提出了一种基于表上作业法的求解算法;分析了制造系统供应链中有潜在利用价值的邮政运输方式问题,在详细描述了该问题的基础上,建立了相应的数学模型,提出了一种近似求解算法,该算法较好地解决了邮政运输方式这样的多种物流、多种运输方式,有严格时限和众多外部约束的特大型、复杂、并行的交连系统的计划调度问题。
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construct: to form by assembling or combing parts; build:建造
be composed of: 由....组成,由....构成 | construct: to form by assembling or combing parts; build 建造 | currently: belonging to the present time 目前