colorimetrically
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CuRF coupounds are based on the combination of deprotonation and ICT mechanisms. The secondary amines conjugated to 1,8-naphthalimide could be deprotonated by Cu2+, and as a result, large red shifts in both absorption and fluorescence spectra were obtained, from which one could sense Cu2+ colorimetrically and ratiometrically.
CuRF 系列则是基于脱氢和ICT原理的结合,铜离子与受体的作用能够诱使共轭氮原子上的氢脱去,共轭氮原子显负电增强了对荧光团的供电能力,从而引起荧光波长的红移,同时也造成了吸收光谱的红移。
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The secondary amine conjugated to 1,8-naphthalimide could be deprotonated by Zn2+, and as a result, large red shifts in both absorption and fluorescence spectra were obtained, from which one could sense Zn2+ colorimetrically and ratiometrically.
锌离子与 ZnRF1-2的作用能够脱去共轭氮原子上的氢,从而使得荧光光谱和吸收光谱发生红移。
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The content of Ti, Nb and Ta are colorimetrically determined by using H_2O_2, acetone-potassium sulfocyanate and pyrogallic acid respectively.
实验中采用了H_2O_2比色测定Ti,用丙酮-硫氰酸钾比色测定铌,用焦性没食子酸比色测定钽。
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To improve detection efficiency and result accuracy, we developed an improved multiplex PCR technique for specific detection of GMO.①The assay strategy involves multiplex PCR amplification of the target with several biotinylated primer pairs in one tube, followed by the hybridization of multiplex PCR products and membrane-bound oligonucleotide probes. The multiplex PCR/probe hybrid is detected colorimetrically using an alkaline phosphatase conjugate and a chromogenic substrate.
在多基因定性检测技术方面,本文在优化并确定了多基因PCR反应条件的基础上,采用了两种方法对多基因PCR产物进行鉴定:①采用共价结合方法将多个探针固定在Biodyne〓C膜上,将5'端标记生物素的多基因PCR产物与膜上的探针进行杂交及显色反应,首次建立了GMO的多基因PCR-膜相杂交检测方法,实现了一个反应体系中同时检测多种转基因成分的目标,提高了检测效率与准确率。
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Methods: Adenosine deaminase was purified by immtino-affinity chromatography, the enzyme activity was assayed colorimetrically by the measurement of ammonia produced over a fixed timeResults: The purified ADA was shown to be a homogeneity by polyacrylamide gel electrophoresis.
用免疫亲和层析法分离纯化人肾ADA,测氨法测定 ADA活性,经SDS-PAGE测定其分子量。