cho

cho的基本解释

abbr.

chorister (教堂的)唱诗班歌手, chosen 精选的

与cho相关的网络例句 [注：此内容来源于网络，仅供参考]

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

ChinaObjective:To study the antitoxic role of vesicular monoamine transporter 2 (VMAT2) in transpgenic Chinese Hamster ovary cell.Methods:With the technology of transgene from PC12 to CHO,MTT reduction assay was used to detect MPP^+ toxic effect on wild type CHO and transgenic CHO.

利用转PC12细胞基因到CHO细胞中形成的转基因CHO细胞（cDNACHO，采用MTT比色法检测1-甲基-4-苯基吡啶离子（MPP^+对CHO细胞野生株（wtCHO和cDNACHO细胞的毒性作用，并观察利血平-VMAT2的特异性阻滞剂对MPP^+毒性作用的影响。

We found that both CHO dhfr-cell and CHO-K1 cell are best suited for glutamine synthetase amplification.

研究表明，在所选择的五种细胞中，CHO～K1细胞和CH0〓细胞是最适合于GS基因扩增系统的宿主细胞。

与cho相关的网络解释 [注：此内容来源于网络，仅供参考]

CHO：胆固醇

35 进 06-1586 谷丙转氨酶(GPT)/丙氨酸氨基转移酶(ALT)检测试剂盒 台湾元生生物科技股份有限公司38 进 06-1633 胆固醇(CHO)检测试剂盒 台湾元生生物科技股份有限公司

CHO：人事总监

行政总监(CAO)不同于首席财务官(CFO)、首席人事总监(CHO). 行政总监必须在董事会或总经理的授权下行使其权利,行使的方式一般是复核、核转或批准,并在自己的职权范围内以命令、决定、允许、建议、劝告、审查指导下属或协助总经理及董事长开展行政工作.

CHO：中国仓鼠卵巢

以期为进一步探讨吗啡抑制催产素合成与释放的机制提供分子学基础. 1.2.1 细胞培养 中国仓鼠卵巢(CHO)细胞培养以含体积分数0.10胎牛血清的PRMI 1640为培养基,在37 ℃、含体积分数0.05二氧化碳的培养箱中培养.

CHO：总胆固醇

氢酶(LDH) 碱性磷酸酶(ALP) 总蛋白(T-PROT) 白蛋白(ALB) 球蛋白(GLB) 白蛋白/球蛋白(A/G) 总胆红素(TB-K) 直接胆红素(DB-K) 间接胆红素(I-BIL) 肌酸激酶(CK) 肌酸激酶同功酶(CK-MB) 血清钙(Ca) 总胆固醇(CHO) 甘油三酯(TG)

CHO：abbr. chinese hamster ovary cells; 中国仓鼠卵巢细胞