chicklings
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One-day-old SPF chicklings were inoculated via intracephalic injection at a dose of 0.03ml per chickling, consisting of 1:100 tissuer virus suspension. And 4 of experimental group and 2 of control group were killed at 1, 3, 5, 10, 14, 20, 25 and 30 days postinfection, respectively. All the sera, the lymphocyte suspension of peripheral blood, thymus, Fabricius bursa and spleen, were collected.
本研究用AEV-NH937株组织毒1:100倍稀释,0.03ml/只脑内接种1日龄SPF雏鸡,分别在攻毒后1、3、5、10、14、20、25和30天迫杀实验攻毒组4只,对照观察组2只,分离血清,制备外周血、胸腺、法氏囊和脾脏的淋巴细胞悬液,同时采集脑组织,10%福尔马林固定,制备石蜡切片,做H.E。
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The purpose of this study, on the base of former research, was to reveal and better understand some molecular pathogenesis of AE, to set up a working model which could be generally used by anther researcher in the field of animal molecular pathology. One-day-old SPF chicklings were inoculated via intracephalic injection at a dose of 0.03ml per chickling, consisting of 1:100 tissuer virus suspension.
本研究用AEV-NH937株组织毒1:100倍稀释,0.03ml/只脑内接种1日龄SPF雏鸡,分别在攻毒后1、3、5、10、14、20、25和30天迫杀实验攻毒组4只,对照观察组2只,分离血清,制备外周血、胸腺、法氏囊和脾脏的淋巴细胞悬液,同时采集脑组织,10%福尔马林固定,制备石蜡切片,做H.E。
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Four hundred 1-day-old Hyline brown laying chicklings were randomly divided into 5 groups with 4 replicates per group of 20 chicklings per replicate.
试验选用了1日龄海兰褐蛋用雏鸡400只,随机分为5组,每组4个重复,每个重复20只鸡。
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The experiment was conducted to study effects of inulin on the growth performance, indices of immune organs and serum immunoglobulin of laying chicklings as well as the performance of inulin which replaced the antibiotics in diets by adding 0.2%, 0.4% and 0.8% of inulin, respectively.
试验旨在通过在蛋用雏鸡日粮中添加0.2%、0.4%和0.8%的菊粉,研究菊粉对蛋雏鸡生长性能、免疫器官指数和血清免疫球蛋白的影响,以及菊粉替代抗生素对蛋雏鸡的应用效果。
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Inulin ; Laying chicklings ; Growth performance ; Immune organs indices ; Serum immunoglobulin
菊粉; 蛋雏鸡;生长性能;免疫器官指数;血清免疫球蛋白
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