canvas primer
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The method is just like the QuikChange site-directed mutagenesis method except for its special primer. This pair of primers is composed of a long primer and a short primer which is central overlapped.Both of the Tm of 5\'sequence in long primer and Tm of 3\'sequence in short primer are above 66℃.The Tm of short primer is almost half of that of long primer.It is convenient to perform insertion or substitution mutation of DNA fragments larger than 20bp,In addition,it is highly applicable in research about the deletion mutation of DNA fragments larger than 2000bp.
我们实验室一直在寻找一种简单高效的可以用于基因的删除、插入及替代突变的方法,通过大量研究和实验发现,可以使用一种基于反向PCR原理,使用全新的引物设计的突变方法——&COP&突变法,该方法采用与QuikChange点突变法相同的操作步骤,其策略是设计一对中心重合的引物,长引物的5\'端序列和3\'端序列的Tm值均不低于66℃,短引物Tm值约为长引物一半,且与长引物中心重合互补。
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Canvas varieties have oil wax canvas, silicone cloth, cotton canvas, PVC waterproof canvas, Oxford cloth, three anti-camp, fire camp, PVC mesh folder , color of the cloth, non-woven, industrial canvas, etc.; canvas sheeting products mainly include travel, freight yard Gabe, open warehouses, machinery and dust mask, ventilation pipe soft connections, Army to use tents, containers Roof side cloth and cord, and then machine cover Peng, floods with protective cloth, offshore platforms operation with wind walls, etc.; other products have nylon ropes, PVC steel wire rope, steel wire rope PVC container, diameter 6 -40mm corns, automobile use bandages, container yard with wind bandages, nylon sling, the old canopy covering demolition patch-free (using hand-held hot-pressing machine) and so on.
帆布品种主要有油蜡帆布、有机硅布、全棉帆布、PVC防水帆布、牛津布、三防帆布、防火帆布、PVC夹网布、彩条布、无纺布、工业帆布等;帆布制品主要有车船蓬布、货场盖布、露天仓库、机器防尘罩、通风管道软连接、军用帐篷、集装箱顶蓬布及侧帘布、接机遮蓬、抗洪救灾用防护布、海上平台作业用挡风墙等;其它产品主要有尼龙绳、PVC钢丝绳、集装箱专用PVC钢丝绳、内径6-40mm鸡眼、汽车用绑带、集装箱堆场用防风绑带、尼龙吊带、旧篷盖布免拆修补等。
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The invention discloses a method to analyze fish genetic information with mitochondria DNA D-loop controlling zone, which comprises the following steps:(1) extracting genom DNA and mitochondria DNA of fish;(2) designing simple primer in fish mitochondria DNA D-loop controlling zone; possessing twenty one basic groups CAC CCY TRR CTC CCA AAG CYA in upstream primer MitD1-F; possessing twenty three basic groups GGT GCG GRK ACT TGC ATG TRT AA in downstream primer MitD1-R;(3) proceeding PCR reaction under the function of primer;(4) choosing agarose jel electrophoresis;(5) checking sequence for augment mtDNA D-loop gene fragment.
本发明公开了一种利用线粒体DNA D-loop控制区进行鱼类遗传信息分析的方法。它包括如下步骤:(1)分别提取鱼类基因组DNA和线粒体DNA;(2)在鱼类线粒体DNA D-loop控制区设计简并引物:其上游引物MitD1-F有21个碱基:CAC CCY TRR CTC CCA AAG CYA;下游引物MitD1-R有23个碱基:GGT GCG GRK ACT TGC ATG TRT AA;(3)在引物的作用下进行PCR反应;(4)琼脂糖凝胶电泳;(5)对扩增的mtDNA D-loop基因片断进行测序。
- 更多网络解释 与canvas primer相关的网络解释 [注:此内容来源于网络,仅供参考]
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primer detonator:起爆雷管
primer charge 点火药 | primer detonator 起爆雷管 | primer 引爆药包
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nitrocellulose primer:硝基底漆
末道底漆 finish primer | 硝基底漆 nitrocellulose primer | 磷化底漆 etch primer
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nitrocellulose primer:磷化底漆
finish primer 硝基底漆 E~Uqn!bt | nitrocellulose primer 磷化底漆 K]!w | etch primer 硝基面漆 <'J]?B- (q