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Methods Germ tube test, chlamydospore test, CHROMagar Candida and API20 kit system were applied to separate non-Candida albicans strains from Candida albicans. Then PCR was used to amplify the internal transcribed spacer region of ribosomal DNA from 4 species of common Pathogenic non-Candida albicans (Candida glabrata, Candida parapsilosis, Candida krusei and Candida tropicalis), and the products were digested with the two restriction endonucleases (Msp Ⅰ and Hae Ⅲ) respectively. Results The four isolates consisted of 15 strains of Candida glabrata (7.50%), 7 strains of Candida parapsilosis (3.50%), 5 strains of Candida krusei (2.50%), 2 strains of Candida tropicalis (1.00%).
首先采用芽管试验、厚壁孢子试验、法国科玛嘉念珠菌显色培养基及API20CAUX酵母菌鉴定系统将分离自VVC患者阴道内的念珠菌菌株鉴定到种，然后采用真菌通用引物将4种常见非白念珠菌（包括光滑念珠菌、近平滑念珠菌、克柔念珠菌和热带念珠菌）进行PCR扩增，并选用MspⅠ和HaeⅢ两种内切酶对扩增产物进行酶切分析。
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METHODS: The different concentrations of natural monoclonal antikeratin antibody IgM 3B4 were incubated with Candida albicans yeast phase suspension on condition that profited germ tube formation of Candida albicans to observe the action of natural monoclonal antikeratin antibody IgM 3B4 to Candida albicans germ tube formation.And the different concentrations of natural monoclonal antikeratin antibody IgM 3B4 were incubated with the mixed suspenions of Candida albicans yeast phase with malpighian cells, human buccal epithelial cells, endothelial cells of fetal umbilical vein, respectively, to observe the action of natural monoclonal antikeratin antibody IgM 3B4 to the adhesion of Candida albicans to malpighian cells, human buccal epithelial cells and endothelial cells of fetal umbilical vein.
将不同浓度的单克隆天然抗角蛋白抗体IgM 3B4分别与白念珠菌酵母相悬液在有利于芽管形成的条件下共同孵育，倒置显微镜下计数白念珠菌总数以及白念珠菌芽管数；将不同浓度的单克隆天然抗角蛋白抗体IgM 3B4与白念珠菌酵母相和人角质形成细胞混悬液、白念珠菌酵母相和人口腔黏膜上皮细胞混悬液、白念珠菌酵母相和胎儿脐静脉内皮细胞混悬液共同孵育，分别计数人角质形成细胞、人口腔黏膜上皮细胞、胎儿脐静脉内皮细胞上粘附的白念珠菌数。
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Keratin antibody IgM 3B4 specifically against Candida albicans in vitro. METHODS: The different concentrations of natural monoclonal anti??keratin antibody IgM 3B4 were incubated with Candida albicans yeast phase suspension on condition that profited germ tube formation of Candida albicans to observe the action of natural monoclonal anti??keratin antibody IgM 3B4 to Candida albicans germ tube formation.And the different concentrations of natural monoclonal anti??keratin antibody IgM 3B4 were incubated with the mixed suspenions of Candida albicans yeast phase with malpighian cells, human buccal epithelial cells, endothelial cells of fetal umbilical vein, respectively, to observe the action of natural monoclonal anti??
将不同浓度的单克隆天然抗角蛋白抗体IgM 3B4分别与白念珠菌酵母相悬液在有利于芽管形成的条件下共同孵育，倒置显微镜下计数白念珠菌总数以及白念珠菌芽管数；将不同浓度的单克隆天然抗角蛋白抗体IgM 3B4与白念珠菌酵母相和人角质形成细胞混悬液、白念珠菌酵母相和人口腔黏膜上皮细胞混悬液、白念珠菌酵母相和胎儿脐静脉内皮细胞混悬液共同孵育，分别计数人角质形成细胞、人口腔黏膜上皮细胞、胎儿脐静脉内皮细胞上粘附的白念珠菌数。
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Candida albicans：白假丝酵母菌
白假丝酵母菌(Candida albicans)又称白色念珠菌,是常见的条件致病性真菌,其既可引起皮肤粘膜的感染,又可致内脏器官及中枢神经的感染[1]. 近年来,随着广谱抗生素、皮质类固醇激素和免疫抑制剂的被广泛应用,白假丝酵母菌感染呈上长趋势,
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Candida albicans：白色念珠菌
[危害] 有些菌对生物或用具是有害的,例如红(Rhodotorula)会生长在浴帘等湿的家具上;白色假丝(或称白色念珠菌)(Candida albicans)会生长在道衬壁等湿润的人类上皮组织.
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Candida albicans：白假丝酵母
产无性孢子:产节孢子:如地霉属(Geotricum)等,产掷孢子如掷孢酵母属 (Sporobolomyces)等;产厚垣孢子如白假丝酵母(Candida albicans)等. 2)有性繁殖:酵母菌以形成子囊和子囊孢子的形式进行有性繁殖.
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Candida albicans：白念珠菌
白念珠菌高铁还原酶frp1基因的功能研究 收藏此文 全部作者: 梁勇 郑雯 魏东盛 邢来君 李明春 第一作者单位: 南开大学生命科学学院微生物学系 论文摘要: 白念珠菌(candida albicans)是一种常见的条件性致病真菌,其获得铁的能力影响细胞的生长和毒力.
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Candida rugosa：皱褶假丝酵母
用聚丙烯酰胺等材料固定皱褶假丝酵母(Candida rugosa)也可以生产L-苹果酸. 据杨廉婉等报道,用这种固定方法,富马酸酶表观活力可达7237μ/g细胞,酶或收率达90%. 包埋6g湿菌体制成的反应柱,在30℃下可以稳定操作2个月,
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