be of no effect

be of no effect的基本解释

-

无效

与be of no effect相关的网络例句 [注：此内容来源于网络，仅供参考]

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

The content of each part follow as: In the first chapter, as start point and base of the paper, this part focuses on the basic study ofdefinition characteristic of NO, and the existing base of NO-E-Commerceenvironment; In the second one, this part studies the theory base of NO comprehensively applying the theories of core competence competent strategy and transaction cost; Chapter three studies the NO from the coordination of NO, and gives the structure clarification and characteristic of NO firstly, at the same time, put forward the concept of Virtual Enterprise Cluster; Based on such conclusion, studies the model of NO from life cyc organization level process and value chain, and operational mode; In Chapter four, a theoretical explanation was addressed on the above structure by modeling NO with game theory and graphic theory; In the fifth chapter, on the bases of analysis of NO operational risks, coordination mechanism of NO was studied by individually modeling the NO without core and NO with core, and then put forward the solution for coordination mechanism of NO; As an important component of coordination mechanism of NO, Chapter six explored some basic concept of trust and importantly put forward the way of how to build trust in NO, especially investigated the supporting function of valid reputation mechanism of NO for the trust building, importantly an operational method on building reputation mechanism and evaluation method in NO were given; The last chapter applied theconclusion of the paper to investigate the famous trade Web-SUNBU.

全文共分为七章，主要内容如下：第一章作为全文的理论出发点和基础，围绕网络组织的定义、特征以及网络组织生存基础--电子商务环境等方面对网络组织的基本概念进行了阐述；第二章综合运用核心能力、竞争战略和交易费用理论对网络组织产生的理论基础进行阐述；第三章首先从组织协调的角度对网络组织进行了研究，给出了网络组织的结构，分类和特征，同时并给出了虚拟企业群簇；然后在此基础上分别研究了网络组织的生命周期模型、层次模型、过程模型、价值链模型，以及运行模式；第四章综合运用博弈论、图论的相关知识，通过构建网络组织的模型，对上一章所研究的网络组织结构的形成机理给出了一种理论解释；第五章在分析网络组织运行风险的基础上，分别建立无盟主网络组织的博弈论模型和有盟主网络组织的博弈论模型，详细研究了网络组织的协调机制，然后给出了网络组织协调机制的解决方案；第六章作为网络组织协调机制的重要组成部分，本章在讨论了网络组织中建立信任机制的必要性的基础上，研究了网络组织信任关系的类型，提出了在网络组织中如何建立信任机制。

Two merging methods of Bézier curves are generalized to tensor product Bé zier surfaces, we propose two methods to approximate two pieces of adjacent tensor product Bézier surfaces by a piece of tensor product Bézier surface. We offer the explicit formulas of the merged tensor product Bézier surfaces with all kinds of merging conditions.

同样，Bézier曲线的两种合并方法也被成功的推广到张量积Bézier曲面上，得到把两片相邻张量积Bézier曲面合并成一片张量积Bézier曲面的两种合并方法，并给出在各种合并条件下的合并曲面控制顶点的显示表示式。

与be of no effect相关的网络解释 [注：此内容来源于网络，仅供参考]

be of no effect：无效

be eager to do sth渴望做某事 | be of no effect无效 | be fond of爱好,喜爱

be of no effect：无效, 没有作用, 不中用

vt.招致, 实现, 达到(目的等) | be of no effect 无效, 没有作用, 不中用 | bring into effect 实行, 实施, 使生效, 实现

to be null and void and of no effect：无效

caused by want of due diligence on the part of sb 由于某人没有克尽职责所引... | to be null and void and of no effect 无效 | goods of an inflammable, explosive or dangerous nature 具有易燃、易爆等危险性质...

make no difference: be of no importance; have no effect：对......不重要,没影响

The applicants were interviewed in turn by the m... | 10. make no difference: be of no importance; have no effect 对......不重要,没影响 | It will make no difference whether the meeting will be held tod...