bast vessel
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The ultrastructure resultshowed that in model group blood intima was adema,broadening, evendiscontinuous and incomplete under blood vessel bast. In the blood themonocyte and the lymphocyte infiltrated to intension board. Manymacrophages could befound and there were massive all levels of dissolve theenzyme body and swallow slightly soak in its cytoblastema,The endosarc netand the ribose body was increased and outside the amnion the pseudopodappeared. Endothelial cell morphogenesis changed. It is obvious thatmitochondria was dropsy, cytoblastema was non-uniformity.
超微结构结果显示模型组血管内皮下内膜水肿、增宽,甚至间断不完整;血中单核细胞及淋巴细胞向内弹力板浸润,可见较多巨噬细胞,其胞浆内含大量各级溶酶体及吞噬小泡,内质网及核糖体增多,胞膜外出现伪足;内皮细胞形态发生改变,可见线粒体水肿,胞浆不均匀;中药治疗后,上述病理损害明显减轻。
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Methods: In order to discuss the JIANGU granule treatment GIO molecular action mechanism, this research takes the osteoporosis sickness animal experimentation model by the glucocorticoids-induced induction domestic rabbit, through HE dyeing research Chinese native medicine JIANGU granule to vacancy bone sunken number influence; Through the immunity histochemistry technology,observes the blood vessel bast growth factor in the bone tissue expression. Through home position hybrid technology,in mRNA member level research, collagen I mRNA distribution and expression.
本研究以糖皮质激素诱导的家兔作为骨质疏松症动物实验模型,通过HE染色研究中药健骨冲剂对骨组织的空缺骨陷窝数的影响;通过免疫组织化学技术,观察血管内皮生长因子在骨组织的表达;通过原位杂交技术,在mRNA分子水平研究,Ⅰ型胶原mRNA在骨组织中分布及表达。