argu
- argu的基本解释
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诐
- 更多网络例句与argu相关的网络例句 [注：此内容来源于网络，仅供参考]
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Recombinant ptotein made up 39.2%(pET-20b-bglA) and 33.5%(pET-28a-bglA) of the total proteins in the intracellular fraction, the solubility proportion of the enzyme up to 32.8%(pET-20b-bglA) and 40.1%(pET-28a-bglA), the activities of the enzyme were 66.8 (pET-20b-bglA) and 71.2 U/mg (pET-28a-bglA). These results showed that E. coli BL21-CodonPlus (DE3)-RIL with argU tRNA, ileY tRNA and leuW tRNA genes helped to improve expression of the enzyme through enhanced identification of the rare codons AGA/AGG, AUA and CUA. Further optimized the conditions for inducing, the solubility proportion of the enzyme was 70.6% at 16 °C and 1.2 times higher than 37 °C. The solubility proportion of the enzyme increased from 12.3% to 32.8% when IPTG concentrations dropped from 1000 μM to 25 μM. The recombinant enzyme was purified by heat treatment, DEAE Sepharose anion-exchange chromatography and TOYOPEARL HW-55F.
maritima MSB8 的-葡萄糖苷酶基因 bglA克隆至表达载体 pET-20b 和 pET-28a，构建重组质粒 pET-20b-bglA 和 pET-28a-bglA，然后转化不同大肠杆菌 E.coli 宿主，Tm-SIGlA 在 E.coli BL21-CodonPlus(DE3)-RIL 中获得高效表达，重组蛋白的表达量为 33.5%(pET-28a-bglA)和 39.2%(pET-20b-bglA)，可溶性比例为 32.8%(pET-20b-bglA)和 40.1%(pET-28a-bglA)，比酶活达 66.8 (pET-20b-bglA)和 71.2 U/mg (pET-28a-bglA)，这些结果表明，E.coli BL21-CodonPlus(DE3)-RIL 宿主带有的 argU tRNA、ileY tRNA 和 leuW tRNA 基因，分别增强对稀有密码子 AGA/AGG、AUA 和 CUA 的识别，有助于提高该酶在 E.coli 中的表达；进一步优化诱导条件，重组 E.coli BL21-CodonPlus(DE3)-RIL/pET-20b-bglA 在 37 ℃下诱导培养，IPTG 浓度由 1000 μM 降至 25 μM，目的蛋白可溶性表达由 12.3%增至 32.8%，提高 1.7 倍，16 ℃下低温诱导实现目的蛋白 70.6%的可溶性表达，较 37 ℃下诱导培养提高 1.2 倍。
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On the other hand, I argu'd with my self, That this really was the way not to deliver my self, but entirely to ruin and destroy my self; for unless I was sure to kill every one that not only should be on Shore at that Time, but that should ever come on Shore afterwards, if but one of them escap'd, to tell their Country People what had happen'd, they would come over again by Thousands to revenge the Death of their Fellows, and 1 should only bring upon my self a certain Destruction, which at present I had no manner of occasion for.
另外，我也认识到，这种主动攻击野人的计划不仅不能拯救自己，反而会完全彻底地毁灭自己。因为，除非我有绝对把握杀死当时上岸的每一个人，还能杀死以后上岸的每一个人；否则，如果有一个人逃回去，把这儿发生的一切告诉他们的同胞，他们就会有成千上万的人过来报仇，我这岂不是自取灭亡吗？这是我当前绝对不应该做的事。
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Argu think When It comes to..., some 2 people e m opposi te 2 revers in both e statemen but....
当说到国际体育比赛，诸如奥林匹克运动会和世界杯足球赛，有些人认为它能增进国与国之间的友善。
- 更多网络解释与argu相关的网络解释 [注：此内容来源于网络，仅供参考]
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The boundary line between the farms was a bone of contention between the two farmers：农场之间的界线是两位农民的争端的起因
1."a bone of contention"=a cause of argu... | The boundary line between the farms was a bone of contention between the two farmers. 农场之间的界线是两位农民的争端的起因. | 2."feel in sb's bones that"=...