acridinium

与acridinium相关的网络例句 [注：此内容来源于网络，仅供参考]

Chemiluminescent immunoassay is one of the advanced immunoassay ofnon-radioisotopic immunoassay because of its high sensitivity, wide dynamic range,high accuracy, stable labeled protein, full automation and extensive application field.CLIA using acridinium ester derivatives as chemiluminescent label has advantages oflow background, high signal-to-noise ratio, no need of catalyst and simpleluminescence system. Different aspects associating the DMAE·NHS-based CLIA were studied in thisthesis, including synthesis of acridinium ester, chemiluminescentcharacteristics of DMAE·NHS, labelling antibody or streptavidin with DMAE·NHS,two-site sandwich chemiluminescent immunoassay for TSH, two-sitesandwich chemiluminescent immunoassay involved biotin-streptavidinsystem for TSH, and competitive chemiluminescent immunoassay usingbiotin-streptavidin system for TT〓. The desired acridinium ester, DMAE·NHS, was synthesized according to thereference method with some modifications. The products were identified by IR, NMR,MS and elemental analysis. In our method, KOH was used in place of NaOH tosynthesize benzyl ester of 3,5-dimethyl-4-hydroxybenzoic acid,2',6'-dimethyl-4'-(n-succinimidyloxycarbonyl) phenyl-acridinium-9-carboxylate waspurified on a silica gel column with chloroform/ethylacetate(4:1, v/v) as eluent andfurther purified by triturating with hexane/acetone(2:1, v/v).

DMAE·NHS的合成是本论文的关键和难点，我们对文献方法进行改进：文献方法用氢氧化钠与3，5-二甲基-4-羟基苯甲酸反应制得3，5-二甲基-4-羟基苯甲酸钠，再用3，5-二甲基-4-羟基苯甲酸钠与苄氯作用制备3，5-二甲基-4-羟基苯甲酸苄酯，我们用氢氧化钾代替氢氧化钠，使合成取得成功；在合成2'，6'-二甲基-4'-苯基-吖啶-9-甲酸酯时，文献方法对粗产品进行两次硅胶柱层析纯化，第一次柱层析用氯仿／乙酸乙酯(4∶1，V∶V)作溶剂和淋洗剂，第二次柱层析用己烷／丙酮(2∶1，V∶V)作溶剂和淋洗剂，按照文献方法得到的不是所需要的化合物，我们只进行第一次柱层析纯化，然后用己烷／丙酮(2∶1，V∶V)进行研磨，过滤，洗涤，除去溶于己烷／丙酮(2∶1，V∶V)的部分，得到所需要的产品。

Four peaks of acridinium of 2 showed pronounced upfield shifts due to the conversion of acridinium to acridane in 5 equiv of either F-(Figure 3b) or AcO-(Figure 3c).

对于与中心吡啶环相邻的质子（ΔHa=1.48ppm）以及相对于吡啶的氮的对位质子（（ΔHc=1.47ppm）观察到了最大的化学位移。

The addition of F-/AcO- to 2 was further confirmed by monitoring the changes in its 1H NMR spectra in the presence of either F- or AcO-(Figure 3). Four peaks of acridinium of 2 showed pronounced upfield shifts due to the conversion of acridinium to acridane in 5 equiv of either F-(Figure 3b) or AcO-(Figure 3c).

F－/AcO－添加到2中通过监测其在存在F－或AcO－时H NMR谱的变化进一步得到确认（图3）。2的吖啶盐的四个峰值证明了由于在5个当量F－（图3b）或AcO－（图3c）中吖啶盐转化成吖啶而引起的明显的高场位移。

与acridinium相关的网络解释 [注：此内容来源于网络，仅供参考]

acridinium oxime：吖啶肟

6.acridine orange 吖啶橙 | 7.acridinium oxime 吖啶肟 | 8.active oxygen 活性氧