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The major results of this proposal are as follows:(1) ABA induces a rapid, substantial accumulation of apoplastic H2O2 in mesophyll and bundle sheath cells of maize leaves, and the accumulation of apoplastic H2O2 is involved in the induction of the chloroplastic and cytosolic antioxidant enzymes.(2) ABA-induced H2O2 production activates a 46 kDa MAPK, which in turn induces the expression of antioxidant genes and up-regulates the activities of antioxidant enzymes. The activation of MAPK also enhances H2O2 production, forming a positive amplification loop.(3) Water stress also induces the activation of a 46 kDa MAPK, which is dependent on the accumulation of ABA and H2O2 production induced by water stress and involved in the up-regulation of the expression and the activities of antioxidant enzymes.(4) ABA-induced H2O2 production mediates NO generation, which in turn activates a 46 kDa MAPK and results in the up-regulation in the expression and the activities of antioxidant enzymes in ABA signaling. NO-independent signaling is also involved in ABA- and H2O2-induced antioxidant defense.
本项目的主要研究结果如下:(1)ABA诱导的H2O2产生主要出现在叶肉细胞与维管束鞘细胞的质外体中,质外体H2O2的积累能够上调叶绿体与细胞溶质中抗氧化酶的活性;(2)ABA诱导的H2O2产生活化一个46kDa的MAPK,由此而导致编码抗氧化防护酶基因的表达与酶活性的上调;而MAPK的活化也能增强H2O2的产生,从而形成一个正的反馈调节环;(3)水分胁迫也能诱导一46kDa MAPK活化,这一MAPK活化依赖于水分胁迫诱导的ABA积累以及H2O2的产生,同时参与水分胁迫诱导的抗氧化防护基因的表达与抗氧化酶活性的上调;(4)ABA诱导一个依赖于H2O2的NO产生,NO活化一个46kDa的MAPK,从而导致抗氧化防护基因的表达以及抗氧化酶活性的上调;同时一个不依赖于NO的信号转导途径也存在于ABA诱导的抗氧化防护过程中。
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This conclusion is based on three groups of experiments with detached root tips of wheat seedlings: By following the time course changes of NO synthase , superoxide synthase and ABA accumulation under dehydration stress, we found that ABA accumulation was preceeded by the changes of NOS and superoxide synthase. ABA accumulation induced by dehydtrion stress and osmotic stress can be blocked by NO scanvegers and NOS inhibitors, while only dehydration stress-induced ABA accumulation can be blocked by ROS scanvegers. Exogenous applied NO or ROS induced ABA accumulation in root tips of wheat seedlings, and synergistic effects were found between NO and NO. The in planta expereiments also proved that ROS and NO are involved in water stress-induced ABA accumulation.Ⅱ.
证据主要来自以离体根尖为材料的实验:(1)通过检测干旱胁迫下小麦根尖NOS活性、O〓合成酶活性和ABA积累的时间变化进程,发现ABA积累滞后于ROS和NO的变化;(2)抑制剂实验表明,NO清除剂和NOS抑制剂可以抑制自然干旱胁迫及渗透胁迫诱导的小麦根尖ABA积累,ROS清除剂抑制自然干旱胁迫诱导的ABA积累而不抑制渗透胁迫诱导的小麦根尖ABA积累;(3)在非胁迫条件下施加外源的ROS或NO可以诱导小麦根尖积累ABA,ROS和NO之间具有协同作用。
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The results implied that NO/ROS-induced ABA accumulation come from de novo synthesis pathway rather than degration of conjugated forms. The protein synthesis inhibitor actidone blocked the NO-induced ABA accumulation while having no effects on ROS-induced ABA accumulation, indicating that the action of NO was gene-expression dependent and that of ROS was gene-expression independent. These results suggested that NO may serve as a stress signal in the regulation of ABA-biosynthesis and ROS may serve as substrate in ABA biosynthesis or be involved in the regulation of enzyme activities.
1ABA合成抑制剂fluridone抑制ROS和NO诱导的ABA积累,表明NO/ROS诱导的ABA积累来源于ABA的从新合成而不是已有结合态ABA的代谢;(2)蛋白质合成抑制剂环己亚胺可以抑制NO诱导的ABA积累而对ROS诱导的ABA积累没有影响,说明NO的作用涉及基因表达而ROS的作用与基因表达无关,暗示NO的作用方式可能是作为一种干旱胁迫信号物质参与ABA合成的调节,而ROS则可能参与了相关酶活性的调节或直接参与了ABA合成的底物反应。
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ABA:脱落酸
[3] 脱落酸,(aba)是植物体内存在的一种天然抑制剂,广泛存在于植物器官组织中. 在将要脱落和休眠的组织器官中含量更高,它与生长素,赤霉素,细胞分裂素的作用是对抗的. 它有抑制萌芽和枝条生长提早结束生长的,
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ABA:美国银行业协会
(十八) 美国银行业协会(ABA)表示,美国第一季消费者贷款违约率由上季的3.22%升至3.23%,按揭贷款违约率由上季的3.03%升至3.52%,透支违约率由上季的1.46%升至1.89%,信用卡违约率由上季的5.52%升至6.60%;ABA首席经济学家James Chessen表示,
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ABA:阿坝
49 日落时间 2009-05-24 19:56:50 邮政编码 618000 今日天气预报 (11:00发布) 生活指数 健康指数 交通指数 户外指数阴 25℃/ 19℃ 东北风小于3级 紫外线...今天是2009年5月24日 星期日 五月初一 阿坝 (Aba) 城市标识图片征集
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ABA:美国银行家协会
(中央社华盛顿2008年10月30日法新电)美国银行家协 会(ABA)今天对政府当局施压银行接受「它们没有申 请且也 不需要」的政府资金援助,表达关切.
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ABA:abbr. absicisic acid; 脱落酸
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