Sacramento perch
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Perch (scientific name: Perca fluviatilis) perch to perch Section species of fish, the common name five black.
河鲈(学名:Perca fluviatilis)为鲈科鲈属的鱼类,俗名五道黑。
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This research is taking sea perch as the material, and cloning the Syntaxin 1B gene. After extracting RNA from sea perch tissue, we gained the first chain of cDNA by RT-PCR. Then designing a pair of primers according to the sequence of Syntaxin 1B from other organism. Based on the first chain of cDNA cloned from sea perch, the partial sequence of Syntaxin 1B gene is been cloned by using TD-PCR and we received the 751bp fragment at last.
本实验从鲈鱼脑组织中提取总RNA,通过反转录得到cDNA第一条链,根据已克隆出的其它生物的Syntaxin 1B基因序列,设计并合成引物,在妒鱼syntaxin1B基因部分序列克隆与分析 cDNA第一条链的基础上使用降落PCR,进行体外扩增妒鱼 Syntaxin IB基因的部分序列,得到长度为75 lbP的片断,在胶中洗脱下该片段,与pMD 18一T载体链接,转化入感受态细胞,挑取阳性克隆扩大培养,提取质粒,经HindIH和x一gaH两内切酶消化得到75 1 bp的片断,证明链接成功。
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After a nine-for-25 shooting effort in a loss Tuesday to Sacramento, he joked that he probably missed the "cowbells and the food poisoning," references to the rowdy Arco Arena crowd that often harassed the Lakers earlier this decade, as well as his infamous bout with food poisoning from hotel room service in Sacramento.
在周二25投9中输给国王后,他开玩笑说自己可能因为&魔咒和食物中毒&的原因所以全无手感,魔咒是指近十年国王的主场总是会给湖人制造不少麻烦,而食物中毒则指萨那门托臭名昭著的酒店服务。
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Sacramento perch:沙克里门投鲈
荐椎 sacral vertebrae | 沙克里门投鲈 Sacramento perch | 荐骨横突系 sacro-transverso-transversalis system