M. L.
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[医] 正中线
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There're big, medium and small bellyband according to the belly measurement so as to achieve ideal waist- line;Size:S/M/L.
男用:本产品采用优质弹力带制成,可使腰腹收缩美观,同时对弯腰工作者有护腰作用,对腰部扭伤者可减轻疼痛,促进愈合;女用:本产品采用优质弹力带制成,按照腰部自然形状,上下稍大,中部细小,长期使用可维持优美体形,特别适用于妇女产后,收缩松弛的腹部,根据腹围大小,可依次用大、中、小号收腹带,以达到理想的腰部曲线;作用:腹部束紧和固定;尺码:S/M/L。
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The methodological 2 oxidation 75 μ Mol/L are cobaltic alone or jointly 30, the Rg3 action of 60 μ G/m L is mixed at 24 H of cell of esophagus cancer EC9706 48 H, part fluid of the Qing Dynasty on collection cell education, detect with ELISA law among them hemal endodermis grows factor level, in order to contain RPM I1640 of serum of 10% embryoes ox education radical does blank to contrast.
方法将75μmol/L的二氧化钴单独或联合30、60μg/m l的Rg3功能于食管癌EC9706细胞24 h和48 h,分别收取细胞培养上清液,用ELISA法检测其中血管内皮生长因子水平,以含10%胎牛血清RPM I1640培养基做空白对照。
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Our study includes four aspects. In the first aspect we study several important conditions of porcine oocytes maturation in vitro and oocytes cleavage after parthenogenetic activation and found mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15% PFF+0.57mMcysteine is a good culture condition .When the Cocs are cultured in it ,the maturation rate and oocytes cleavage rate are higher than those of foreign covered. Our result are (86.7±3.35)% and (86.3±4.16)% and the highest report of foreign is(85.7±4.1)%.In the second aspect we study the effect of different chemical activations on development of porcine parthennogenetic embryo and found two best activation method. The first one is that putting the maturation MII oocytes in the 20μmol/L ionomycin for 30 minutes and then putting them in the NCSU-23 condition containing 5μg/mICB and 5mM/L6-DMAP for 3.5 hours, the oocytes cleavage rate and morulae/blastocysts development rate are (76.7±7.6)% and (37.1±6.4)%.The second one is that putting the maturation MII oocytes in the 200μM/L Thimerosal for 20 minutes and then putting them in the NCSU-23 condition containing 8mM DTT for 30 minutes
本研究分为4个部分,第一部分对影响猪卵母细胞体外成熟和孤雌激活后胚胎分裂的几个重要条件进行了比较研究,确立了一种较好的培养方法:与颗粒细胞共培养,找到了一种适合猪卵母细胞体外成熟的培养基:mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15%PFF+0.57mM半胱氨酸,成熟率和分裂率分别为(86.7±3.35)%和(86.3±4.16)%,国外报道的最高成熟率为(85.7±4.1)%;第二部分对猪卵母细胞孤雌激活的化学方法进行了研究,确立了化学激活猪卵母细胞的两种最佳方法:1将成熟的去卵丘颗粒细胞的MII期卵母细胞用20μmol/Lionomycin作用30min,再将卵母细胞培养于含5μg/mlCB和5mM/L 6-DMAP(6-二甲基氨基嘌呤)的NCSU-23培养液中,卵裂率和桑囊胚发育率达到(76.7±7.6)%和(37.1±6.4)%2将成熟的去卵丘颗粒细胞的MII期卵母细胞在200μM/L的Thimerosal中处理20min,再与8mM的DTT共孵育30min,卵裂率和桑/囊胚形成率为(81.0±2.8)%和(39.6±2.7)%;第三部分对孤雌激活胚胎的培养条件进行了研究,确立了一种最佳的胚胎培养条件:在SOF简单培养基中添加颗粒细胞进行前3天的培养,然后转入添加胎牛血清的NCSU—23培养基并和输卵管上皮细胞进行后期的培养,其桑椹胚和囊胚的发育率为(59.5±3.2)%;第四部分研究了IGF-I
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I. M. L. loyd:劳埃德
劳埃德 E. J. L. loyd | 劳埃德 I. M. L. loyd | 劳积勋 R. P. L. Froc
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Cereal Grains M L Eastridge and J L Firkins:谷物类
精饲料 CONCENTRATE FEEDS | 谷物类 Cereal Grains M L Eastridge and J L Firkins | 油籽和油籽粉 Oilseeds and Oilseed Meals J K Bernard
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bar l m. l:酒吧
isla f. 岛屿,小岛 | bar l m. l 酒吧 | Roma (地名) 罗马