Listeria
- Listeria的基本解释
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[医] 李司忒氏菌属, 利斯塔氏菌属
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in order to establish a rapid, sensitive and specific detection method for identification of four kinds of food-borne pathogenic bacteria including salmonellla spp, listeria monocytogenes, staphylococcus aureus and bacillus cereus by using multiplex pcr, according to invasion protein a geneof salmonellla spp, transcriptional regulatory protein gene of listeria monocytogenes, autolysin gene ofstaphylococcus aureus and gyrase b subunit gene of bacillus cereus, four pairs of specific primers were designed for multiplex pcr amplification.
小 摘要:为建立一种利用多重pcr技术检测和鉴定沙门氏菌、单核细胞增生李斯特菌、金黄色葡萄球菌和蜡样芽孢杆菌的方法,根据沙门氏菌侵袭力蛋白a基因、单核细胞增生李斯特菌蛋白转录调控基因、金黄色葡萄球菌自溶素基因、蜡样芽孢杆菌的促旋酶b亚单位基因设计引物,进行多重pcr扩增,并对多重pcr反应体系进行优化。
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The method is based on real time reverse-transcription-PCR amplification of mRNA to detect viable Listeria monocytogenes by specific hlyA gene. This method can overcome the false-positive caused by amplification of nonviable Listeria monocytogenes in the detection process.
为克服传统PCR检测的假阳性问题,有效的检测单增李斯特活菌,本研究提取单增李斯特菌的总RNA并进行反转录,以单增李斯特菌的必要毒力基因hlyA为靶基因,设计特异性引物和TaqMan探针进行实时PCR检测,研究了检测的特异性和灵敏度,考查了对人工污染牛乳进行检测的应用性。
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This paper isolated and identified Listeria monocytogenes by the first step of the physiologic and biochemical tests from 1627 samples. 1627 random samples from 9 sorts of quick-frozen food, aquatic products, resh meat, meat protucts, fruit, vegetables, bean product, raw milk, salted product were collected from supermarkets and peasant-trade mart and some faeces of animal and person. The total detectable rate of Listeria monocytogenes was 14.5%. The results showed that the detectable rate of Listeria monocytogenes were higher. It expressed that pollution level of Listeria monocytogenes in Baoding and circumjacent area was taller.
本文对采自速冻食品、水产品、生肉、熟肉制品、水果、蔬菜、豆制品、奶及奶制品、腌制品等9类食品及动物粪便和人源粪便共1627份样品进行了单增李斯特菌的分离与鉴定,单增李斯特菌的总检出率为14.5%,表明单增李斯特菌的检出率偏高,在9类食品样品中,生肉中单增李斯特菌的检出率最高,高达27.4%,其次是水产品,检出率为24.7%,应引起高度重视,以免引起食源性疾病的暴发。
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Listeria ivanovii:依氏利斯特菌
Listeria ivanovii 伊氏利斯特菌 | Listeria ivanovii 依氏利斯特菌 | Listeria monocytogenes 单核细胞增生利斯特菌
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Listeria ivanovii:伊氏利斯特菌
Listeria innocua 无害利斯特菌 | Listeria ivanovii 伊氏利斯特菌 | Listeria ivanovii 依氏利斯特菌
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Listeria welshimeri:威氏利斯特菌
Listeria spp 利斯特菌属某些种 | Listeria welshimeri 威氏利斯特菌 | Listeria welshimeri 魏氏利斯特菌