January 15 on the lunar calendar
- January 15 on the lunar calendar的基本解释
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元宵节
- 相似词
- 更多 网络例句 与January 15 on the lunar calendar相关的网络例句 [注:此内容来源于网络,仅供参考]
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BEGIN MILESTONES ===== 0x945e3c8 2008/03/11 17:02:35.7189: debug log dumped due to signal 11 ===== END MILESTONES ========== BEGIN RING BUFFER ===== 0x945e3c8 2008/03/11 14:41:03.1538: window 0x9510840 open location: old="", new="x-nautilus-desktop:" 0x945e3c8 2008/03/11 14:41:07.4960: finished loading window 0x9510840: x-nautilus-desktop: 0x945e3c8 2008/03/11 15:44:48.0181: selection changed in window 0x9510840 x-nautilus-desktop:///trash 0x945e3c8 2008/03/11 15:44:52.1769: selection changed in window 0x9510840 x-nautilus-desktop:///home 0x945e3c8 2008/03/11 15:45:50.2681: directory view open_location window=0xb1f02890: file:///home/heng/%E4%B8%8B%E8%BD%BD 0x945e3c8 2008/03/11 15:45:50.2681: window 0xb1f02890 open location: old="file:///home/heng", new="file:///home/heng/%E4%B8%8B%E8%BD%BD" 0x945e3c8 2008/03/11 15:45:50.2860: window 0xb1f02960 open location: old="", new="file:///home/heng/%E4%B8%8B%E8%BD%BD" 0x945e3c8 2008/03/11 15:45:50.2864: present NEW spatial window=0xb1f02960: file:///home/heng/%E4%B8%8B%E8%BD%BD 0x945e3c8 2008/03/11 15:45:50.6858: finished loading window 0xb1f02960: file:///home/heng/%E4%B8%8B%E8%BD%BD 0x945e3c8 2008/03/11 15:45:51.2669: selection changed in window 0xb1f02960 file:///home/heng/%E4%B8%8B%E8%BD%BD/temp.rar 0x945e3c8 2008/03/11 15:45:53.4393: delete files: file:///home/heng/%E4%B8%8B%E8%BD%BD/temp.rar 0x945e3c8 2008/03/11 15:45:53.6466: selection changed in window 0xb1f02960 0x945e3c8 2008/03/11 15:48:31.7278: selection changed in window 0xb1f02960 file:///home/heng/%E4%B8%8B%E8%BD%BD/temp.rar 0x945e3c8 2008/03/11 15:53:50.3606: fm_directory_view_activate_files window=0x9510840 x-nautilus-desktop:///home 0x945e3c8 2008/03/11 15:53:50.3658: directory view open_location window=0x9510840: file:///home/heng 0x945e3c8 2008/03/11 15:53:50.3659: window 0x9510840 open location: old="x-nautilus-desktop:", new="file:///home/heng" 0x945e3c8 2008/03/11 15:53:50.3660: present EXISTING spatial window=0xb1f02890: file:///home/heng 0x945e3c8 2008/03/11 15:53:52.2887: fm_directory_view_activate_files window=0xb1f02890 file:///home/heng/%E4%B8%8B%E8%BD%BD 0x945e3c8 2008/03/11 15:53:52.2891: directory view ===== END RING BUFFER ===== This configuration for the debug log can be re-created by putting the following in ~/nautilus-debug-log.conf (use ';' to separate domain names): max lines=1000
开始里程碑===== 0x945e3c8 2008/03/11 17:02:35.7189 :调试日志倾倒由于信号11日=====完里程碑==========开始环形缓冲区===== 0x945e3c8 2008/03/11 14:41:03.1538 :窗口0x9510840开放地点:旧="",新=的" X鹦鹉螺-桌面说:" 0x945e3c8 2008/03/11 14:41:07.4960 :成品装载窗口0x9510840 :的X鹦鹉螺-桌面: 0x945e3c8 2008/03/11 15:44:48.0181 :选择改变窗口0x9510840 的X鹦鹉螺-桌面:///垃圾桶 0x945e3c8 2008/03/11 15:44:52.1769 :选择改变窗口0x9510840 的X鹦鹉螺-桌面:///主页 0x945e3c8 2008/03/11 15:45:50.2681 :目录浏览open_location窗口= 0xb1f02890 :档案:///家/恒/%类% b8 % 8B条%,并可%屋宇署%屋宇署 0x945e3c8 2008/03/11 15:45:50.2681 :窗口0xb1f02890开放地点:旧="档案:///主页/衡",新="档案:///家/恒/%类% b8 % 8B条%,并可%屋宇署%屋宇署" 0x945e3c8 2008/03/11 15:45:50.2860 :窗口0xb1f02960开放地点:旧="",新="档案:///家/恒/%类% b8 % 8B条%,并可%屋宇署%屋宇署" 0x945e3c8 2008/03/11 15:45:50.2864 :当前新的空间窗口= 0xb1f02960 :档案:///家/恒/%类% b8 % 8B条%,并可%屋宇署%屋宇署 0x945e3c8 2008/03/11 15:45:50.6858 :成品装载窗口0xb1f02960 :档案:///家/恒/%类% b8 % 8B条%,并可%屋宇署%屋宇署 0x945e3c8 2008/03/11 15:45:51.2669 :选择改变窗口0xb1f02960 档案:///家/恒/%类% b8 % 8B条%,并可%屋宇署%屋宇署/ temp.rar 0x945e3c8 2008/03/11 15:45:53.4393 :删除档案:档案:///家/恒/%类% b8 % 8B条%,并可%屋宇署%屋宇署/ temp.rar 0x945e3c8 2008/03/11 15:45:53.6466 :选择改变窗口0xb1f02960 0x945e3c8 2008/03/11 15:48:31.7278 :选择改变窗口0xb1f02960 档案:///家/恒/%类% b8 % 8B条%,并可%屋宇署%屋宇署/ temp.rar 0x945e3c8 2008/03/11 15:53:50.3606: fm_directory_view_activate_files窗口= 0x9510840 的X鹦鹉螺-桌面:///主页 0x945e3c8 2008/03/11 15:53:50.3658 :目录浏览open_location窗口= 0x9510840 :档案:///主页/珩 0x945e3c8 2008/03/11 15:53:50.3659 :窗口0x9510840开放地点:旧="的X鹦鹉螺-桌面:",新="档案:///家/恒" 0x945e3c8 2008/03/11 15:53:50.3660 :目前现有的空间窗口= 0xb1f02890 :档案:///主页/珩 0x945e3c8 2008/03/11 15:53:52.2887: fm_directory_view_activate_files窗口= 0xb1f02890 档案:///家/恒/%类% b8 % 8B条%,并可%屋宇署%屋宇署 0x945e3c8 2008/03/11 15:53:52.2891 :目录浏览=====完环形缓冲区=====此配置为调试日志,可以再创造把下列/鹦鹉螺-调试- log.conf (使用';'分开域名):最大值线= 1000 我帮你翻译了这么多,你才给我5分,我亏死了,再给我加30还差不多可以不下次我可以帮你的
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Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.
参考已发表的ChIL-15基因序列,设计合成引物,应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA,将其与SmalⅠ酶切处理的pUC19载体连接,构建重组质粒pUC19ChIL-15;用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因,与pMD 18-T载体相连构建重组质粒pMDChIL-15;然后用KpnⅠ/PstⅠ双酶切下mChIL-15基因片段,定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中,构建重组质粒pP_RoChIL-15,对其测序确认读框正确后,转入大肠杆菌DH5α中诱导表达并进行纯化,用重组ChIL-15(rChIL-15)免疫豚鼠,制备多克隆抗体;再用HindⅢ/PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因,定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中,经酶切、PCR和序列测定鉴定后,与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞,经三轮蚀斑纯化,构建重组杆状病毒rBac-ChIL-15,用该重组病毒感染处于对数生长期的Sf9细胞,按不同的时间收取样品,离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。
- 更多网络解释 与January 15 on the lunar calendar相关的网络解释 [注:此内容来源于网络,仅供参考]
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lunar calendar Uiy:农历
春节 The Spring Festival Uiy | 农历 lunar calendar Uiy | 正月 lunar January; the first month by lunar calendar Uiy
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the Gregorian calendar/solar calendar:阳历
阴历lunar calendar/Chinese Lunar Calendar | 阳历the Gregorian calendar/solar calendar | 月亮女神,邪恶女神Luna
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circumlunar satellite:绕月卫星
绕月卫星 circumlunar satellite | 嫦娥1号 Chang'e-1 lunar probe; Chang'e-1 lunar satellite | 月球探测卫星 lunar probe; lunar exploration satellite; lunar orbiter