- 相关中文词汇
- 蒸煮
- 拼写相近单词
- Digby
- Digenea
- Digitaria
- Digitigrada
- Digynia
- 更多网络例句与Dig.相关的网络例句 [注：此内容来源于网络，仅供参考]
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CHARGES. At this time DIG charges no brokerage fees or commissions. DIG may charge for incidental banking-related fees such as wire charges for deposits/withdrawals and returned check fees. DIG reserves the right to change its fee structure at any time without notice. Fees do not currency but may in the
27.2 客户同意由于客户未能完全和实时履行客户协议规定义务或因客户提供的信息不真实、不准确导致DIG受到的损失，造成的支出包括律师费用等向DIG做出赔偿。
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Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.
将PCR产物进行重组，并将阳性重组质粒，应用M13测序系统对产物进行DNA序列分析，并与MPM-129株P1基因核苷酸进行同源性比较，发现有4个位置的碱基发生了变化，其同源性为98.8％，证实了PCR所扩增DNA片段的准确性和特异性，同时也证实了不同MP组型的P1基因即使在保守区也存在着一定的差异，将克隆的目的DNA片段用异羟基洋地黄毒苷配基用随机引物法标记制备MP DNA探针，杂交后用碱性磷酸酶标记的抗Dig多克隆抗体与杂交体反应，再用BCIP和NBT呈色，制备MP DNA探针，鉴定所扩增片段的特异性，与同位素探针比较，Dig探针不受半衰期限制，可反复使用，而且价格低廉，值得推广使用。
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Enynes with an aryl ring at the alkyne give 2,3,9,9a-tetrahydro-1H-cyclopentanaphthalenes by a 5-exo-dig cyclization followed by a Friedel-Crafts-type ring expansion.
带有芳环的1，6-二烯体和炔作用，并通过5-exo-dig路径环化，再紧接着Friedel-Crafts扩环，可以得到2，3，9，9a-四羟基-1H-环戊二烯并萘。6-endo-dig途径的环化在一些例子中作为副反应被观察到，尽管，这在一些具体的反应中，是作为主反应出现的。
- 更多网络解释与Dig.相关的网络解释 [注：此内容来源于网络，仅供参考]
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DIG DUG：打空气
打空气 (Dig Dug) 下载[FC中文游戏]☉打空气 (Dig Dug) 下载[FC中文游戏]来自网络收集或网友上传,版权归打空气 (Dig Dug) 下载[FC中文游戏]原作者所有. ☉如果遇到打空气 (Dig Dug) 下载[FC中文游戏]总是不能下载的请与我们联系,谢谢合作!!
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DIG DUG：挖金子
挖金子 (Dig Dug) 美版 磁碟机版 .挖金子 (Dig Dug) 美版 磁碟机版 [fc游戏]挖金子 (Dig Dug) 美版 磁碟机版 [fc游戏]点下面下载: 挖金子 (Dig Dug) 美版 磁碟机版 [0]
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DIG VOX：设置DIG模式下声控电路的增益
038 DIG MODE, 选择DIG(数传)的工作模式和边带 | 039 DIG SHIFT, 定义DIG���式下的载频偏移 | 040 DIG VOX,设置DIG���式下声控电路的增益