Cricetulus

Cricetulus的基本解释

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[医] 田鼠属

与Cricetulus相关的网络例句 [注：此内容来源于网络，仅供参考]

Food habits were studied for four dominant rodents (Phodopus roborovskii, Dipus sagitta, Meriones meridianus and Cricetulus barabensis) inhabiting in Ordos sandland, NeiMongol in 1997. The results obtained by three methods of feeding experiments, analysis of cheek pouch contents, and analysis of stomach contents showed no significant differences basically.

采用笼养饲喂法、野外个体颊囊内含物统计法和胃内含物分析法3种方法，于1997年对内蒙古鄂尔多斯沙地中的4种主要啮齿动物（小毛足鼠Phodopus roborovskii、三趾跳鼠Dipus sagitta、子午沙鼠Meriones meridianus和黑线仓鼠Cricetulus barabensis）的食性进行了研究。3种方法得出的结果基本一致。

The Markov forecast model, time series method (triple exponential smoothing method and a combined of triple exponential smoothing method and Markov model) and stepwise regression forecast method were choosed, and the basic principles and processes of the three models were analyzed and compared. Based on above, the three methods were used to predict population dynamic of Cricetulus barabansis and Meriones unguiculatus. The characteristic, accuracy and applicability of three methods were analysed and compared. The results showed that,(1) Markov model belongs to a middle-long time forecast model, and the result was interval forecast, but it reached the requirement of forecasting the harmful population dynamic of rodents. Through the forecasted population dynamic of Cricetulus barabansis and Meriones unguiculatus in 2004, the results were accurate; this meant that Markov model was a good forecast model to Cricetulus barabansis and Meriones unguiculatus population.

选择马尔可夫链预测模型、时间序列分析法(三次指数平滑法、三次指数平滑法和马尔可夫链预测模型的组合预测法)和逐步回归预测法，对这三种模型的基本原理、模型的构建过程进行对比分析，在此基础上用此三种方法预测了黑线仓鼠和长爪沙鼠的种群数量，并对三种方法的特点、预测的准确度和适用范围进行了比较分析，结果表明：(1)马尔可夫链预测模型属于中长期预测，预测的结果为区间预测，但对于害鼠种群数量的预测来说已经达到要求，通过对2004年黑线仓鼠和长爪沙鼠种群数量的预测，预测的结果都比较准确，这说明对于预测黑线仓鼠和长爪沙鼠种群的数量动态，马尔可夫链模型是比较好的模型。

The RT-PCR product was inserted into pTG19-T vector and transformed into E. coli successfully. By blastn, the sequence results of Kunming mus musculus were in complete accordance with the conservative sequence of Genbank NR_003278 (791bp-1153bp). By Blastn in NCBI, the sequence with little difference among animals was confirmed to be conservative. After Blastn, fourteen complete CDS coding for different animals were chosen. According to VECTOR NIT 9.0 software, the similarities between Kunming mus musculus and bos taurus, homo sapiens, erinaceus europaeus, cricetulus griseus, sus scrofa, dasypus novemcinctus, rattus norvegicus, rabbit, equus caballus, macaca fascicularis, didelphis virginiana, monodelphis domestica and vombatus ursinus was 67%, 100%, 100%, 36%, 100%, 100%, 67%, 100%, 100%, 92%, 99%, 99% and 99%. In the phylogenetic tress constructed with the forteen 18S rRNA by Treeview, the Kunming mus musculus clustered with cricetulus griseus, sus scrofa and rabbit, which was nearer to cricetulus griseus and was most far away from macaca fascicularis.(3) After sencodary structure analyses of 18S rRNA of mus musculus, an oligonucleotide fragment for RNAi was designed and synthesized, which was transformed into plasmid, and restriction enzyme analyses and sequencing results should the expression plasmid pGPH1/ GFP/Neo-mouse-sh 18S rRNA were constructed for RNAi successfully.

结果①通过RT-PCR检测显示18S rRNA基因在小鼠卵巢组织和单个GV期、MⅠ期卵母细胞中均有表达，且在未成熟卵母细胞中，MⅠ期的表达明显强于GV期的表达；②RT-PCR产物克隆测序结果显示：昆明小鼠18S rRNA基因保守区序列与基因库序列[NR_003278保守区部分(791bp～1153bp)]完全一致；Blastn比对结果发现：在不同物种中差异较小，选出14种生物18S rRNA全序列经VECTOR NIT 9.0软件分析，提示昆明小鼠18SrRNA与牛、人类、刺猬、中国仓鼠、猪、犰狳、褐鼠、兔子、马、食蟹猴、负鼠、短尾猊、袋熊的18S rRNA的相似率依次为67%，100%，100%，36%，100%，100%，67%，100%，100%，92%，99%，99%，99%；Clustal 1.81和Treeview构建出的分子进化树表明：在上述14种生物中昆明小鼠与中国仓鼠进化关系最近，与兔子、猪聚成一簇，与食蟹猴进化关系最远；③根据18S rRNA二级结构设计并合成RNA干扰寡核苷酸片段，重组质粒经过限制性内切酶及测序表明成功构建了pGPH1/GFP/Neo-mouse-sh 18S rRNA干扰表达质粒。