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objective to establish immunological methods specific for detecting antigens in different groups of monoclonal antibodies.methods indirect immnofluorescence assay was applied to identify specificity of the two groups of monoclonal antibodies prepared with crude antigen and recombinant antigen of aspergillus fumigatus,respectively.two different double monoclonal antibody sandwich elisa assays established with the two groups of antibodies were performed to detect antigents in the cell culture supermatants of 19 common species of aspergillus,penicillium marneffei,and 5 species of candidas.results the results of indirect immnofluorescence assay indicated that the monoclonal antibodies prepared with crude antigen of aspergillus fumigatus were specific for antigens in both clinical isolates and environmental isolates of aspergillus, whereas the other group of monoclonal antibodies was proved to be specific for aspergillus fumigatus of both clinical and environmental isolates.the elisa assay established with the crude antigen-specfic monoclonal antibodies could detect both of the clinical and environmental isolates of aspergllius, while the other assay could only detect aspergillus fumigatus of both clinical and environmental isolates.and no cross reaction with the cell culture of penialllium marneffei and candidas was observed with the two methods.conclusion the elisa assays can detect both of the clinical and environmental isolates of aspergillus,and differentiate aspergillus fumigatus from other species of aspergillus.
目的 用2组曲霉单克隆抗体建立特异性识别不同种类曲霉抗原的检测方法。方法采用天然烟曲霉抗原免疫，获得广谱针对曲霉抗原的单克隆抗体；采用重组烟曲霉抗原获得特异性针对烟曲霉抗原的单克隆抗体，用间接免疫荧光鉴定，并分别建立2种双抗体夹心elisa法，对19种常见的环境和临床分离曲霉株、马尔尼菲氏青霉菌及念珠菌培养液进行检测。结果间接免疫荧光显示，用天然烟曲霉抗原免疫获得的单克隆抗体(mabs-1)可广谱识别多种曲霉分离株，而重组烟曲霉抗原获得的单克降抗体(mabs-2)仅能特异性结合临床和环境分离的烟曲霉抗原。用mabs-1建立的双抗体夹心elisa法可检测19种常见曲霉株培养液；用特异性针对烟曲霉抗原单克降抗体(mabs-2)建立的双抗体夹心elisa法可特异性检测临床和环境分离株烟曲霉培养液；与其他曲霉株无交叉反应；2种双抗体夹心elisa法与马尔尼菲氏青霉菌及念珠菌培养液均无交叉反应。
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Methods BALB/c mice were immunized by cytoderm antigen of Aspergillus fumigatus, excreting antigen and inactivated conidiospore respectively, to prepare monoclonal antibodies specific to Aspergillus. Cross-reactions between mAbs and antigens from Aspergillus or Monilia genus were characterized by immunofluorescence.
采用烟曲霉细胞壁抗原、分泌抗原和灭活分生孢子，分别免疫BALB/c小鼠，制备特异性的抗曲霉单克隆抗体，免疫荧光法鉴定单克隆抗体与曲霉属和念珠属抗原的交叉反应。
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A strain library containing 226 bacterial and fungal xylanase producers was successfully constructed, two bacterial producers Pseudomonas sp WXULI-11 and WL-11 and three fungal producers Aspergillus niger XY04B, Aspergillus niger XY07C and Aspergillus glaucus XY12D were screened out from this library.
建立了一个包括226株细菌和霉菌木聚糖酶产生菌的小型菌种库，从中筛选出了2株细菌（Pseudomonas sp WXULI-11和WL-11）及3株霉菌（Aspergillus nigerXY04B、Aspergillus niger XY07C及Aspergillus glaucus XY12D），它们的共同点是木聚糖酶生物合成量高，且不产或少产纤维素酶。
- 更多网络解释 与Aspergillus相关的网络解释 [注：此内容来源于网络，仅供参考]
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Aspergillus terreus：土曲霉+Aspergillus flavus 黄曲霉
-Aspergillus restrictus 局限曲霉+Aspergillus candidus 亮白曲霉 | -Aspergillus terreus 土曲霉+Aspergillus flavus 黄曲霉 | acclimatization 气候适应
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Aspergillus glaucus：灰绿曲霉+ascorbic acid 抗坏血酸
-Aspergillus fumigatus 烟曲霉+ascorbate 抗坏血酸盐 | -Aspergillus glaucus 灰绿曲霉+ascorbic acid 抗坏血酸 | -Aspergillus nidulans 构巢曲霉+Aspergillus 曲霉属
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黄曲霉+Ascomycetes 子囊菌纲：Aspergillus flavus
-Aspergillus candidus 亮白曲霉+Ascaris vermicularis 蛲虫 | -Aspergillus flavus 黄曲霉+Ascomycetes 子囊菌纲 | -Aspergillus fumigatus 烟曲霉+ascorbate 抗坏血酸盐