All broke into a loud laugh.
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哄堂大笑
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Broke estate wind water, a five favorable because, first, home of the popular wave is advancing wave upon wave, the wave of tidehead Guanwaidichan as one has not been 关内 Nanshan "substitutes" have been contained, rather because work gets big cake, enjoy more Gospel; Second, the department kilometer momentum to continue sweeping, that the current direction is still a certain potential to be tapped village, but village has water reserves for the development of land, and only broke with the village close to successfully enjoy Phuket real estate situation favorable to the extension; Third, broke a vast reserve of land and 11 million square metres of land available for the real estate industry highly competent, and broke the natural environment unique, for the development of high-quality flats for the rich soil; Fourth, Shenzhen, Guangzhou, Zhongshan and Zhejiang, and other well-known developers have about broke, overseas Chinese,"Guangzhou Biguiyuan,""Zhongshan Garden Music" will be successfully settled in that the supply of real estate market is rapidly broke assembly, formed; Fifth, further spread kilometer race, For kilometer property kilometer race with the actual contribution to local economic development, then broke to "miss"!
2000年横岗地产风声水起,得缘于五大利好,其一,全民置业化浪潮一浪高过一浪,关外地产作为这一浪潮中的潮头之一,并未因关内南山&替代品&的出现受到抑制,反倒因为蛋糕越做越大,享受到更多福音;其二,布吉片区发展势头继续势如破竹,显示目前布吉方向仍有一定潜力可挖,但是布吉可供开发土地储备已经告急,而横岗与布吉只是咫尺之遥,使横岗可望享受到布吉地产形势利好的延伸;其三,横岗土地储备丰厚,1100万平方米土地可供地产界游刃有余,同时,横岗自然环境得天独厚,为开发高素质楼盘预备了丰厚的土壤;其四,深圳、广州、中山及浙江等地知名地产商纷纷看好横岗,中国海外、&广州碧桂园&、&中山雅居乐&等即将在横岗落脚,表明横岗地产供应市场正在迅速集结、形成;其五,片区竞赛进一步蔓延,片区竞赛对于片区地产的实际贡献与对于当地经济的发展有目共睹,使横岗不愿再&错失良机&!
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The effects and mechanism of GABAergic neurons, NOergic neurons, opioid peptide and cyclic adenosine monophosphate in the nucleus reticularis thalami on sleep-wakefulness cycle of rats and the effects and mechanism of the 5-HTergic nerve fibers project from the nucleus raphes dorsalis to RT on sleep-wakefulness cycle of rats were investigated with the methods of brain stereotaxic, nucleus spile, microinjection and polysomngraphy.1. The effects of GABAergic neurons in RT on sleep-wakefulness cycle of rats1.1 Microinjection of 3-mercaptopropionic acid (3-MP, a kind of glutamate decarboxylase inhibitor) into RT. On the day of microinjection, sleep only decreased a litter. On the second day, sleep marked decreased and wakefulness marked increased. On the third and fourth day, sleep and wakefulness stages resumed to normal.1.2 Microinjection of gamma-amino butyric acid (GABA 1.0μg) into RT enhanced sleep and reduced wakefulness compared with control; while microinjection of L-glutamate (L-Glu, 0.2μg) decreased sleep and increased wakefulness; microinjection of bicuculline (BIC, 1.0μg), a GABAA receptor antagonist, enhanced wakefulness and reduced sleep; microinjection of baclofen (BAC, 1.0μg), GABAB receptor agonist, had the same effects as GABA.2. The effects of NOergic neurons in RT on sleep-wakefulness cycle of rats2.1 Microinjection of L-arginine (L-Arg, 0.5μg) into RT decreased sleep compared with control, but there were on statistaical difference between L-Arg group and control; while microinjection of sodium nitroprusside (SNP, 0.2μg), a NO donor into RT, sleep marked decreased and wakefulness marked increased. Microinjection of nitric oxide synthase inhibitor, N-nitro-L-arginine (L-NNA, 2.0μg) into RT enhanced sleep and reduced wakefulness.2.2 After simultaneous microinjection of L-NNA (2.0μg) and SNP (0.2μg) into RT, SNP abolished the sleep-promoting effect of L-NNA compared with L-NNA group; after simultaneous microinjection of L-NNA (2.0μg) and L-Arg(0.5μg) into RT, we found that L-NNA could not blocked the wakefulness-promoting effect of L-Arg.3. The effects of opioid peptide in RT on sleep-wakefulness cycle of rats3.1 Microinjection of morphine sulfate (MOR, 1.0μg) into RT increased wakefulness and decreased sleep compared with control; while microinjection of naloxone hydrochloride (NAL, 1.0μg), the antagonist of opiate receptors, into RT, enhanced sleep and reduced wakefulness.3.2 After simultaneous microinjection of MOR (1.0μg) and NAL (1.0μg) into RT, the wakefulness-promoting effect of MOR and the sleep-promoting effect of NAL were not observed compared with control.4. The effects of cAMP in RT on sleep-wakefulness cycle of rats Microinjection of cAMP (1.0μg) into RT increased sleep and decreased wakefulness compared with control; microinjection of methylene blue (MB,1.0μg) into RT enhanced sleep and reduced wakefulness compared with control.5. The effects of the 5-HTergic nerve fibers project from DRN to RT on sleep-wakefulness cycle of rats5.1 When L-Glu (0.2μg) was microinjected into DRN and normal sodium (NS,1.0μg) was microinjected into bilateral RT. We found that sleep was decreased and wakefulness was increased compared with control; when L-Glu (0.2μg) was microinjected into DRN and methysergide (MS,1.0μg), a non-selective 5-HT antagonist, was microinjected into bilateral RT, We found that sleep was enhanced and wakefulness was reduced compared with L-Glu group.5.2 When p-chlorophenylalanine (PCPA, 10μg) was microinjected into DRN and NS (1.0μg) was microinjected into bilateral RT, We found that sleep was increased and wakefulness was decreased compared with control; microinjection of 5-hydroxytryptaphan (5-HTP, 1.0μg), which can convert to 5-HT by the enzyme tryptophane hydroxylase and enhance 5-HT into bilateral RT, could block the effect of microinjection of PCPA into DRN on sleep-wakefulness cycle.
本研究采用脑立体定位、核团插管、微量注射、多导睡眠描记等方法,研究丘脑网状核(nucleus reticularis thalami,RT)中γ-氨基丁酸(gamma-amino butyric acid ,GABA)能神经元、一氧化氮(nitrogen monoxidum,NO)能神经元、阿片肽类神经递质、环一磷酸腺苷(cyclic adenosine monophosphate,cAMP)及中缝背核(nucleus raphes dorsalis,DRN)至RT的5-羟色胺(5-hydroxytryptamine,5-HT)能神经纤维投射对大鼠睡眠-觉醒周期的影响及其作用机制。1 RT内GABA能神经元对大鼠睡眠-觉醒周期的影响1.1大鼠RT内微量注射GABA合成关键酶抑制剂3-巯基丙酸(3-MP,5μg),注射当天睡眠时间略有减少,第二日睡眠时间显著减少,觉醒时间明显增多,第三、四日睡眠和觉醒时间逐渐恢复至正常。1.2大鼠RT内微量注射GABA受体激动剂GABA( 1.0μg)后,与生理盐水组比较,睡眠时间增加,觉醒时间减少;而RT内微量注射L-谷氨酸(glutamic acid, L-Glu, 0.2μg)后,睡眠时间减少,觉醒时间增加;RT内微量注射GABAA受体阻断剂荷包牡丹碱(bicuculline,BIC,1.0μg)后,睡眠时间减少,觉醒时间增加;RT内微量注射GABAB受体激动剂氯苯氨丁酸(baclofen,BAC,1.0μg)后,产生了与GABA相似的促睡眠效果。2 RT内NO能神经元对大鼠睡眠-觉醒周期的影响2.1大鼠RT内微量注射NO的前体L-精氨酸(L-Arg,0.5μg)后,与生理盐水组对比,睡眠时间略有减少,但无显著性意义;而RT内微量注射NO的供体硝普钠(Sodium Nitroprusside,SNP,0.2μg)后可明显增加觉醒时间,缩短睡眠时间;微量注射一氧化氮合酶抑制剂L-硝基精氨酸(L-arginine,L-NNA,2.0μg)后,引起睡眠时间增多,觉醒时间减少。2.2大鼠RT内同时微量注射L-NNA(2.0μg)和SNP(0.2μg)后与L-NNA组比较发现SNP逆转了L-NNA的促睡眠作用;RT内同时微量注射L-NNA(2.0μg)和L-Arg(0.5μg)后,与L-NNA(2.0μg)组比较发现L-Arg可以增加觉醒而缩短睡眠,其促觉醒作用未能被NOS的抑制剂L-NNA所逆转。3 RT内阿片肽对大鼠睡眠-觉醒周期的影响3.1大鼠RT内微量注射硫酸吗啡(morphine sulfate,MOR,1.0μg)后与生理盐水组对比,睡眠时间减少而觉醒时间增加; RT内微量注射阿片肽受体拮抗剂盐酸纳洛酮(naloxone hydrochloride,NAL,1.0μg)后与生理盐水组比较,睡眠时间增加而觉醒时间减少。3.2大鼠RT内同时微量注射MOR(1.0μg)和NAL(1.0μg)后,与生理盐水组对比,原有的MOR促觉醒效果和NAL的促睡眠效果都没有表现。4 RT内环一磷酸腺苷信使对大鼠睡眠-觉醒周期的影响大鼠RT内微量注射cAMP(1.0μg)后与NS(1.0μg)组比较,睡眠时间增多而觉醒时间减少;RT内微量注射亚甲蓝(methylene blue,MB,1.0μg)后,与NS组比较,睡眠时间增多而觉醒时间减少。5中缝背核投射到丘脑网状核的5-羟色胺能神经纤维对大鼠睡眠-觉醒周期的影响5.1大鼠DRN内微量注射L-Glu(0.2μg),同时在双侧RT内微量注射NS (1.0μg)后,与对照组(DRN和双侧RT注射NS, 0.2μg)比较,睡眠时间减少,觉醒时间增多;大鼠DRN内微量注射L-Glu(0.2μg),同时在双侧RT内微量注射二甲基麦角新碱(methysergide, MS, 1.0μg )后,与对照组(DRN注射L-Glu 0.2μg,双侧RT注射NS 1.0μg)比较,睡眠时间增多,觉醒时间减少。5.2大鼠DRN内微量注射对氯苯丙氨酸(p-chlorophenylalanine,PCPA,10μg),同时在双侧RT内微量注射NS (1.0μg)后,与对照组(DRN和双侧RT注射NS, 1.0μg)比较,睡眠时间增多,觉醒时间减少;大鼠DRN内微量注射PCPA(10μg),产生睡眠增多效应后,在双侧RT内微量注射5-羟色胺酸(5-hydroxytryptaphan , 5-HTP, 1.0μg )后,与对照组(DRN注射PCPA 10μg,双侧RT注射NS 1.0μg)比较,睡眠时间减少,觉醒时间增多。
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In love with a broken heart -think I leave today, I cover it all this way- You fell in love with a broken heart -every earthly breath, a lifeless testimony- You fell in love with a broken heart -another lifeless testimony Oh please machine, let's keep this terminal clean I'll see your face in every cloud Down a Dewar's laugh out loud Fly girl shouts to keep it down Paddy's wagon draws a crowd I don't save days I think...leave days God grant me the strength to accept the things I can change And change them I cant miss you In love with a broken heart Face in every cloud, makes me laugh out loud- You fell in love with a broken heart Shouts to keep it down, wagon draws a crowd- In love with a broken heart (Don't you know) Lifeless testimony You fell in love with a broken heart Lifeless testimony In love with a broken heart In these modern times, what your first class said, Time to throw us down, medicine's side effects
爱心都碎了,想我离开,我今天涵盖所有这种方式,你爱上了心都碎了,每一个人间呼吸,没有生命的证词,你爱上了心都碎了,另一个生命的证词噢,请机器,让我们保持这种终端清洁我去看看你的脸在每个云一个杜瓦的哈哈大笑粉煤灰女孩喊道保持下来水稻的车皮提请人群我不保存天我想。。。离开天上帝保佑我的力量,接受的东西我可以改变并更改我不能错过你爱心都碎了面对每云,让我哈哈大笑,你爱上了心都碎了喊声保持下来,货车吸引了人群爱心都碎了生命的证词你爱上了心都碎了生命的证词爱心都碎了在这些现代化的时候,你的一流说,时间把我们失望,药品的副作用
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He speaks loud and clear:他说话响亮清楚
loud 响亮的,吵闹的 | He speaks loud and clear. 他说话响亮清楚. | loud laugh 高声大笑
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stony-broke:身无分文, 一文不名
stone broke 身无分文, 一文不名 | stony broke 身无分文, 一文不名 | [USA]flat broke flat-broke 身无分文, 一文不名
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laugh like a drain / laugh one's head off:(长时间)大笑
嘲笑;讥笑 laugh at | (长时间)大笑 laugh like a drain / laugh one's head off | 对某事一笑了之 laugh off