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RbcL gene was obtained by polymerase chain reaction and cloned into pDM19-T vector. The positive clone identified by PCR was sequenced. The nucleotide sequence was 1266 bp long, including an ORF of 1031bp, encoding a putative 343 amino acids. The deduced amino acid sequence had a high identity above 94.13% with that of tobacco, spinach, sweet potato, corn, Arabidopsis thaliana, Oryza sativa, Vitis vinifera, Marchantia polymorpha and Citrus paradise.

阳性克隆鉴定后进行测序，序列分析结果表明：该基因片断长为1266 bp，包括1031 bp的编码区序列，编码343个氨基酸；其编码区氨基酸与烟草、菠菜、玉米、甘薯、拟南芥、水稻、葡萄、地钱和葡萄柚等9个物种的同源性94.13%以上，并构建了它们间的亲缘关系树。

Taurine is one of the most abundant free amino acids in the mammalian central nervous system and has many putative neurophysiological functions.

牛磺酸是哺乳动物中枢神经系统中含量最为丰富的自由氨基酸之一，具有许多认定的神经生理功能。

The bioinformatics analysis of the deduced amino acid sequences of four genes indicated they are classical AGPs. They contain an N-terminal signal sequence, a central domain rich in Ala, Ser, Thr, Pro/Hyp, and C-terminal hydrophobic transmembrane domain. All of them include two AGP-like domains(rich in noncontinuous Pro residues) and a putative C-terminal GPI anchor signal sequence.

对四个基因编码的蛋白序列进行生物信息学分析，发现它们都包含N端信号肽序列，具有富含Ala、Ser、Thr、Pro／Hyp的长度可变区以及C端疏水跨膜区，还具有两个富含非连续Pro的AGP-like domain，C端存在可能的GPI锚定区。

Expression and processing of putative nonstructural proteins of hepatitis C virus in insect cells using baculovirus vector［J］.

我国丙型肝炎病毒补血河北株基因组的克隆及cDNA全序列的测序与分析［J］。

In particular, the nonsynonymous substitutions at the putative peptide-binding sites are considered to be associated with immunological specificity of MHC B-LBⅡ molecule in Chinese native chickens.

分析认为，那些发生在多肽结合位点上的氨基酸错义替换与鸡MHC B-LBⅡ分子的免疫特异性有关。

These results imply that the putative progenitor cells/stem cells residing in dental papillae can differentiate into odontoblasts under appropriate conditions in vitro.

本研究结果表明牙乳头中的祖细胞或干细胞在体外适宜环境下可分化为成牙本质细胞。

The pregrowth hormone peptide of 204aa deduced from LcGH cDNA included a putative signal peptide (17aa) locating in its N-terminal.

此cDNA全长992nt，其5'端非编码区长93nt，开放阅读框(Open reading frame，ORF)长615nt，3'端非编码区长224nt。

I research the procedure of offer in compromise,such as the claim of offer in compromise,putative taxation,amount change of forfeit,coordinating liability,onus propend,protect of trust,informal negotiation base on factual administration of tax department.

本文针对税务约谈的程序及效力，如约谈请求权、推计课税、罚款增减、协力义务、举证责任、信赖保护、非正式协商性行为等等，以税务机关的实际运用为基础做了进一步研究。

The somatic hybrids usually showed specific bands and combined all the bands of both parents. In combinations, some new bands were detected, and occasionally specific bands of parents were lost, which indicated that chromosome recombination and exchange happened after fusion. Additionally, in some combinations, specific bands from leaf parent were detected, which revealed the plants were of putative hybrid origin, because leaf protoplast cannot redifferentiate by both single culture and co-culture.

体细胞杂种一般表现为具有双亲的特征带，且综合双亲的所有带，但在部分杂种中检测到了双亲都没有的新带，也发现有丢失亲本的特征带或共有带的现象，说明融合后染色体发生了重组和交换；有的引物只检测到叶肉亲本的特征带，根据柑橘叶肉细胞无论单独培养还是共培养均不能再生的事实，可推测其为体细胞杂种或二倍体叶肉亲本型胞质杂种。

Homology analysis showed that the amino acid sequence between putative peptides from above S gene fragments and that from S-RNase of rosaceous plant were 67%-96% of homology.Almond should be assigned to plum subspecific groups with cherry and apricot.

同源性分析表明，获得的10个氨基酸序列与蔷薇科中李亚科S-RNase的氨基酸序列同源性达67%～96%，扁桃与樱桃、杏应同属于李亚科。

More direct, directer ; most direct, directest

径直的，笔直的

Do you know how to use a chain saw?

你知道如何使用链锯吗？